Regulation of Gene Transcription: Control of Transcription

Question 1

How is unregulated activity determined?

Answer 1

• Sequence of -10 & -35 region determine affinity of RNA-P for the promoter
• Doesn’t give mechanism to trun on/off genes or alter level of transcription based on conds.

Question 2

Transcription Factors

Answer 2

• Regulatory proteins
• Ensure a gene is expressed at right time at correct level

Question 3

Type of regulation - Negative Control

Answer 3

• Promoter is active in absense of regulatory protein
• Binds to promoter + stops RNA-P from initiating transcription - Repressor

Question 4

Type of regulation - Positive Control

Answer 4

• Promotor is inactive in absense of regulatory protein
• Regulatory protein binds the promotor + helps RNA-P initiate transcription - Activator

Question 5

Negative Control Regulation - Inducible transcription

Answer 5

Requires an inducer that binds and inactivates the repressor