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MAVM: Microbiology > Removal Exam > Flashcards

Flashcards in Removal Exam Deck (43):
1

Amino acids used in the test for decarboxylase:

Lysine, Arginine, Ornithine

2

Testing 3 amino acids requires 4 tubes, because:

1 tube is needed for control

3

Enzyme that removes the amino group (NH2) from an amino acid:

Deaminase

4

Organisms that produce urease do what to the medium?

Liquefy the medium

5

Hydrogen sulfide production, other than in TSI agar, requires:

An organic source of sulfure, and a source of metal

6

Negative result in Malonate utilization test:

Green and Yellow

7

Gelatinase breaks down gelatin to:

Amino acids

8

(+) DNAse result after 0.1N HCl is added to the plate:

Agar clears around the colony

9

Urine culture:
Gram (-) rod
Motile
TSI: A/A + gas
Urease (-)
IMVC: +/+/-/-

Escherichia coli

10

Blood culture:
Gram (-) red
Motile, no capsule
TSI: A/A + gas
Urease (-)
IMVC: -/-/+/+

Enterobacter cloacae

11

Blood culture:
Gram (-) rod
Urease (+)
PAD (+)
TSI: K/A + gas
LDC (-), ODC (+)

Morganella morganii

12

Stool culture:
Gram (-) rod
Urease (-)
PAD (-)
TSI: K/A
Nonmotile
LDC (-)

Shigella species

13

Allows viability of acid production by oxidizers in oxidation fermentation (OF) medium:

Peptone content is high

14

Stool culture:
Gram (-) rod
Urease (-)
PAD (-)
TSI: K/A + gas + H2S
Motile
LDC (+)
Indole (+)

Aeromonas hydrophilia

15

Oxidase (+):

Aeromonas hydrophilia
Vibrio cholerae

16

Reagent used for string test:

0.5% sodium desoxycholate

17

Indole (+)
Nitrate reduction (+) is also:

Cholera red (+)

18

Number of oxidation fermentation (OF) media are inoculated and what precautions are taken:

2 tubes, one covered with oil so air is excluded

19

Reason for Thioglycollate broth be boiled for 10 minutes before being used:

To drive off oxygen

20

Clostridia that produces a double zone of hemolysis around colonies on BA:

Clostridium perfringens

21

Mycoplasma pneumoniae is incubated:

Aerobically, in a sealed container in carbon dioxide

22

Urease (+) for Ureaplasma is indicated by a:

Brown halo surrounding colonies

23

Organism that procudes no haze in broth culture:

Ureaplasma urealyticum

24

Serological test done for Chlamydia trachomatis on genital smears:

Direct FA using monoclonal Abs

25

Erythema chronicum migrans (ECM):

Cardinal sign of Lyme disease at site of tick bite

26

Broad spectrum antibiotics:

Acts agrains Gram (-), Gram (+), bacterial, and non-bacterial pathogens

27

Resistance to an antibiotic can be transferred from a resistant to a susceptible organism by:

Extrachromosomal plasmid

28

In Kirby-Bauer ST, the 0.5 MacFarland standard is used to:

Adjust the turbidity of the inoculum

29

After inoculating the MH plates for the KBST, duration should the plates dry before adding the disks:

3 to 5 minutes but not > 15 minutes

30

Measurement of the zone of growth of inhibition for the KBST:

On the underside of the plate
With unaided eye
Using ruler, caliper, or template

31

Too much moisture on the surface of the MH plate when performing KBST:

Smaller zone of growth

32

Best indicators of poor storage:

Penicillin and Methicillin

33

In MH agar, it causes increased resistance of P. aeruginosa to aminoglycosides:

Increased Ca and Mg

34

Two concentric zones around the disk in ST with sulfonamides:

Measure the diameter of the outer zone

35

Lowest concentration of drug that will kill all but the minimum defined proportion of viable organisms after incubation for a fixed time under a given set of conditions:

Minimum Lethal Concentration and Minimum Bactericidal Concentration

36

QC when monitoring reagents, how often should reagent disks be checked:

When container is first opened and weekly

37

How often should catalase, oxidase, and coagulase reagents be tested:

Daily and when vial is first opened

38

Record temperatures of incubators, water baths, heating blocks, refrigerators, freezers, and thermometers:

Every use, at the beginning of each day, at the end of each day

39

Check the face velocity of safety cabinets each:

Month

40

A tube of semisolid medium that has resazurin incorporated in it appears pink. Organism expected to be recovered:

Gardnerella vaginalis

41

True about Clostridia:

Botulism is caused by ingesting preformed toxin and can be prevented by boiling food prior to eating;
Pseudomembranous colitis is due to a toxin produced by C. difficile;
Clinically significant Clostridia are found in the normal flora of the colon and in the soil

42

Anaerobic Gram (-) rod can be presumptively ID by its G/S morphology, growth in the presence of bile, and inhibition by a 1 ug Kanamycin disk:

Fusobacterium nucleatum

43

Purulent material from a cerebral abscess was submitted to the laboratory for smear and culture. On direct G/S, Gram (+) cocci in chains and Gram (-) bacilli with pointed ends were seen. Culture results form this specimen will not be available for 48 hours. Therefore, on the basis of the organisms seen on the smear, what is the most likely presumptive ID of the etiologic anaerobic agents?

Fusobacterium species and Peptostreptococcus species