Replication / Transcription

Question 1

An okazaki fragment is

Answer 1

Segment of DNA that participates in the synthesis of the lagging strand

Question 2

DNA polymerase

Answer 2

Synthesise 5’-3’/ Exonuclease activity both side (proof reading)

Question 3

At which stage replication is going on ?

Answer 3

S

Question 4

Processivity is defined as

Answer 4

The average number of nucleotides added before a polymerase dissociates

Question 5

What means fidelity ?

Answer 5

Exonuclease activity/DNA repair systems/Correction base pairing

Question 6

Transcription

Answer 6

Use a DNA strands as template/more selective than replication/produce RNA molecules

Question 7

ARN pol

Answer 7

Can synthesise chains with no primers

Question 8

Differential RNA may result in

Answer 8

production of 2 distinct proteins from a single gene

Question 9

Processing a primary mRNA transcript in a eukaryotic cell does normally involve

Answer 9

Splicing out of introns

Question 10

Best description promoters in E.coli

Answer 10

Promoters contain consensus sequences with the highest affinity for ARN pol

Question 11

What need the DNA pol III to begin the replication

Answer 11

DNA Template + A primer

Question 12

The replication is

Answer 12

Semiconservative/ Bidirectional/ Semi discontinuous

Question 13

OriC

Answer 13

Initiation of the replication at this unique point

Question 14

Nuclease

Answer 14

Enzymes that degrades nucleic acids (Exo/Endo)

Question 15

DNA pol III

Answer 15

Replicative enzyme / High processivity / proofreading (3’-5’)

Question 16

DNA pol I

Answer 16

DNA repair / removal primers / less processivity

Question 17

Helicase

Answer 17

Separation of the 2 parents strand

Question 18

Topoisomerase

Answer 18

Relax topologic stress

Question 19

SSB

Answer 19

Stabilize DNA strand

Question 20

Primase

Answer 20

RNA primer synthesis

Question 21

DNA ligase

Answer 21

Seal the nicks

Question 22

Steps of the replication

Answer 22

Initiation/ Elongation/Termination

Question 23

Telomerase

Answer 23

Reverse transcriptase/ RNA dependant Active in germ cells and totipotent cells

Question 24

Transcriptome

Answer 24

Sum of all RNA molecules produced in a cell under a given set of conditions

Question 25

Transcription requires

Answer 25

DNA templates (promoter) + RNA polymerase + Transcription factors

Question 26

Transcription promoter

Answer 26

before the coding sequences/consensus sequences recognised by RNA pol/TATA box (-30)

Question 27

Maturation process

Answer 27

5'-cap addition + 3'-poly(A) tail addition + Splicing out introns

Question 28

Alternative processing

Answer 28

Process in which a particular exon may or may not be included into the mature mRNA transcript