Reporter Genes

Question 1

What makes a good reporter gene?

Answer 1

1) easily visible and quantifiable
2) endogenous - does not interfere with the cell’s normal function
3) it has to be some kind of foreign piece of DNA

Question 2

what is transient transfection?

Answer 2

the constructed plasmids are introduced into mammalian cells in some ways, but the foreign genes of the plasmid are not integrated into the genome of the mammalian cell. With the growth and division of cells, foreign genes will be gradually and totally lost