required practical 1: effect of named variable on the rate of enzyme controlled reactions Flashcards
examples of variables that affect the rate of reaction
enzyme concentration/volume
substrate concentration/volume
temperature
pH
how is temperature controlled
use a water bath
monitor using thermometer at regular intervals
how is pH controlled
use a buffer solution
use a pH meter
describe a control experiment
use denatured enzymes
everything else should be the same
how is the rate of an enzyme controlled reaction measured
measure time taken for set point to be reached
1/time
what is a safety risk in this experiment and how is it reduced
handling enzymes may cause reaction, wear gloves and eye protection
why is using a colorimeter to measure colour change better than comparing to standards
not subjective and more accurate
how can this data be represented on a graph
independent variable on x axis and rate on the y axis
why will rate of reaction decrease overtime
initial rate is highest as substrate concentration is not limiting - the most enzyme substrate complexes form
reaction slows as substrate is used up
