required pratical: effect of ph on amylase

Question 1

1

Answer 1

one drop of iodine solution into one well of the spotting tile

Question 1

2

Answer 1

3 test tubes- 2cm cubed of starch, 2cm cubed of amylase solution, 2cm cubed ph 5 buffer solution

Question 2

3

Answer 2

place all 3 test tubes in a water bath, leave for 10 minutes so they can reach right temp

Question 3

4

Answer 3

combine all 3 solution into one test tube- mix with stirring rod, return to water bath and start a stopwatch

Question 4

5

Answer 4

after 30 secs- use stirring rod to transfer one drop of solution in a well in the spotting tile that contains iodine

Question 5

6

Answer 5

iodine should turn blue-black showing precence of starch

Question 6

7

Answer 6

take sample every 30 secs - continue untill stays orange - tells us starch is no longer present

Question 7

8

Answer 7

repeat experiment several times using different ph buffers

Question 8

problem 1

Answer 8

taking sample every 30 secs - only have aprox time each reaction is complete- ever 10 secs

Question 9

problem 2

Answer 9

looking for iodine not going blue-black- not obvious - gradual colour change - several people to look at when the reaction is complete