Requirement for Microbial Growth

Question 1

2 types of bacterial requirement

Answer 1

Physical and Chemical

Question 2

three primary groups of temperature

Answer 2

psychrophiles, mesophiles, and thermophiles

Question 3

(cold-loving microbes) at 0 degree celsius

Answer 3

Psychrophiles

Question 4

(moderate - temperature- loving microbes) - 25-40 degree celsius, are the most common type of microbes

Answer 4

Mesophiles

Question 5

(heat-loving microbes) - 50-60 degree celsius

Answer 5

Thermophiles

Question 5

have an optimum growth temperature of 80 degree celsius or higher

Answer 5

Hypothermophiles

Question 6

121 degree celsius and above

Answer 6

Extreme thermophiles

Question 7

refers to acidity or alkalinity of a solution

Answer 7

pH

Question 8

what narrow pH range does most bacteria grows best ?

Answer 8

range near neutrality between pH 6.5 and pH7.5

Question 9

Grow at high pH environment

Answer 9

Alkaliphiles

Question 10

considered as an extremophiles that grow in acidic environment below pH4

Answer 10

Acidophiles

Question 11

orce that water exert on the semi-permeable membrane (plasma membrane) surrounding the cell.

Answer 11

Osmotic Pressures

Question 12

Water moves across the plasma membrane in response to an unequal distribution of dissolved solutes in the environment.

Answer 12

Osmotic Pressures

Question 13

salt concentration inside is high

Answer 13

Hypertonics solution

Question 14

does bacteria need hypertonics or isotonic solution?

Answer 14

isotonic solution

Question 15

are microorganisms that require certain concentration of salt to survive

Answer 15

Halophiles

Question 16

three types of halophiles

Answer 16

Extreme halophiles, Obligate Halophiles, Facultative Halophiles

Question 17

require high salt concentration

Answer 17

Extreme halophiles

Question 18

require 30% of salt for growth

Answer 18

Obligate Halophiles

Question 19

requires 15% of salt for growth

Answer 19

Facultative halophiles

Question 20

organisms that require oxygen to live

Answer 20

Obligate aerobe

Question 21

3 types of Physical requirements

Answer 21

Temperature, pH, Osmotic Pressure

Question 22

5 types of Chemical requirements

Answer 22

1. Carbon
2. Sulfur, Nitrogen, and Phosporus
3. Trace elements such as Iron, Copper, Molybdenum, and Zinc
4. Organic growth Factors
5. Oxygen

Question 23

have the ability to grow in the absence of oxygen.

Answer 23

Facultative anaerobe

Question 24

bacteria that are unable to use molecular oxygen for energy-yielding reactions.

Answer 24

Obligate anaerobe

Question 25

cannot use oxygen for growth, but they tolerate it fairly well

Answer 25

Aerotolerant anaerobes

Question 26

they are aerobic but they grow only in oxygen concentrations lower than loose in air.

Answer 26

Microaerophile

Question 27

bacteria that grow on the body. Does Not cause disease or infection

Answer 27

Normal flora

Question 28

it is necessary to change carbon dioxide gas from the air and water around them into a sugar called glucose. 

Answer 28

Carbon

Question 29

are necessary for protein and nucleic acid biosynthesis

Answer 29

Sulfur, Nitrogen, and Phosporus

Question 30

Most microbes get these elements by degrading proteins and nucleic acids, but some capture nitrogen gas or ammonia or get sulfur from other ions in the environment. 

Answer 30

Sulfur, Nitrogen, and Phosporus

Question 31

Organic compounds such as amino acids, purines, pyrimidines, and vitamins 

Answer 31

Organic growth Factors

Question 32

6 conditions necessary for bacterial growth 

Answer 32

FATTOM Food Acidity Time Temperature Oxygen Moisture 

Question 33

it is an increase in the number of bacteria in a population rather than in the size of individual cells. 

Answer 33

Bacterial Growth

Question 34

growth whose rate becomes more rapid in proportion to the growing total number in size 

Answer 34

Exponential Growth

Question 34

it is the division of a single entity into two or more parts and the generation of those parts to separate entities resembling the original

Answer 34

Binary Fission

Question 35

formula for Generation Time

Answer 35

G = t / n

Question 36

represents the number of live cells in a bacterial population over a period of time.

Answer 36

Bacterial Growth Curve

Question 37

5 "i"method in culturing microbes

Answer 37

Inoculation Isolation  Incubation  Inspection Identification 

Question 38

introducing bacteria into a culture media for microbial growth  Producing a pure culture  Introduce bacteria into a growth medium using “aseptic technique” to prevent contamination

Answer 38

Inoculation

Question 39

colony on media, one kind of microbe pure culture 

Answer 39

Isolation 

Question 40

2 types of culture

Answer 40

Pure culture and Mix culture

Question 41

growing microbes under proper conditions  Allow organisms to grow under the optimal conditions 

Answer 41

Incubation

Question 42

an instrument used in the production of an anaerobic environment.

Answer 42

Anaerobic jar

Question 43

use of data, correlation, to ID organism to exact species  Correlating data from all observations to ID organism to species 

Answer 43

Identification 

Question 44

observation and description of characteristic (data) Colony morphology, microscopic examination (grams stain)  Systematic recording of “DATA”

Answer 44

Inspection