requirements

Question 1

Determine solute concentration in a sample using a spectrophotometer and a
standard curve

Answer 1

measure absorbance
plot the curve (absorbance y axis, concentration x axis)
equation of line y(absorbance) = m(slope)x(concentration) + b (y intercept)

Question 2

Determine solute concentration in a sample using a spectrophotometer and a
regression equation that’s provided

Answer 2

measure absorbance
rearrange the equation
x = (y - b)/m
y = absorbance
x = concentration

Question 3

determine ph of a solution using a ph meter

Answer 3

dip the ph meter in the solution and wait for the number to come up

Question 4

determine the pOh of a solution using a ph meter

Answer 4

take the ph and subtract that number from 14

Question 5

Distinguish among acidic, basic, and neutral solutions in terms of pH

Answer 5

acidic starts from 1 and goes to 7 (neutral) from 7 it goes to 14 basic

Question 6

Determine [H + ] in a solution

Answer 6

high H+ = low pH

Question 7

Determine [OH - ] in a solution

Answer 7

[OH − ]=10 ^−pOH

Question 8

Prepare a percent by volume solution (C 1 V 1 = C 2 V2 )

Answer 8

C1 = Initial concentration of the stock solution (in % by volume)
V1 = Volume of the stock solution you need to use (in mL or L)
C2 = Desired concentration of the final solution (in % by volume)
V2 = Total volume of the final solution (in mL or L)

Question 9

Prepare a percent solution

Answer 9

PercentbyVolume= TotalVolumeofSolution(mL) VolumeofSolute(mL) ​ ×100

Question 10

Measure volume using a graduated cylinder, serological pipette, and/or a
micropipette

Question 11

Interpret information that is stamped on a serological pipette

Question 12

Relate enzyme concentration, substrate concentration, pH, and temperature to activity of an enzyme

Answer 12

Enzyme activity goes up as enzyme concentration, substrate concentration (to a point), and temperature goes up. Enzyme activity is optimal at certain pH levels

Question 13

Determine activity of an enzyme from a regression equation that’s obtained by plotting data in Microsoft Excel

Answer 13

y = mx+b ; m = enzyme activity

Question 14

Determine respiration rate of yeast cells by plotting data in Microsoft Excel

Question 15

Determine activity of an enzyme by plotting time vs absorbance data in Microsoft
Excel

Question 16

Determine respiration rate of yeast cells from a regression equation that’s obtained
by plotting data in Microsoft Excel

Answer 16

y = mx+b ; m = respiration rate

Question 17

Measure percent Transmittance (%T) for a sample using a spectrophotometer

Question 18

Determine proportion (percentage) of dividing cells in a medium from counts
obtained using a hemocytometer

Answer 18

proportion of dividing cells = number of dividing cells / total number of cells

Question 19

Determine OD 600 value for a sample using a spectrophotometer

Question 20

Distinguish between viable and nonviable cells after staining with methylene blue
solution

Answer 20

viable cells are clear and nonviable are blue

Question 21

Determine cell concentration in a yeast medium using a hemocytometer and calculate percent cell viability in the medium

Question 22

Identify differences in cell division among yeast, animal, and plant cells

Answer 22

yeast cells divide by budding, animal cells divide with a cleavage furrow, and plant cells divide by forming a cell plate

Question 23

Determine chlorophyll a, chlorophyll b, and carotenoid concentrations in a sample
from spectrophotometer readings

Answer 23

concentration = absorbance / Molar absorptivity coefficient * 10 mm

Question 24

Relate Hardy-Weinberg equilibrium principle to allele frequencies in a population

Answer 24

p+q=1

Question 25

Relate effect of population size on persistence time of an allele in a population

Question 26

Relate effect of heterozygote advantage on persistence time of an allele in a population

Question 27

Relate effect of increasing or decreasing fitness of an allele on persistence time of the allele in a population

Question 28

Calculate allele frequencies from genotype frequencies (see questions at the end of the Modeling Evolution lab)

Question 29

Calculate frequency of genotypes from allele frequencies (see questions at the end of the Modeling Evolution lab)

Answer 29

p+q=1 p = frequency of the dominant alelle q = frequency of the recessive alelle

Question 30

Be familiar with all the information that’s in each of the relevant lab exercises in the manual