Respiration (3) Flashcards

(59 cards)

1
Q

Aerobic respiration creates about ____ ATP

A

30-32 ATP

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2
Q

Respiration occurs in which 4 processes?

A

Glycolysis, Acetyl Coa formation, Krebs cycle, Oxidative phosphorylation

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3
Q

Where does glycolysis occur?

A

cytoplasm

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4
Q

Where does Acetyl CoA formation occur?

A

mitochondria

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5
Q

Where does Krebs cycle occur?

A

mainly matrix

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6
Q

Where does oxidative phosphorylation occur?

A

inner membrane of mitochondria

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7
Q

Reduction definition

A

loss of oxygen or gain of electrons

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8
Q

Oxidation definition

A

gain of oxygen or loss of electrons

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9
Q

Respiration is an (oxidation/reduction) process

A

oxidation

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10
Q

A reducing agent (accepts/donates) hydrogen/electrons

A

donates e-

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11
Q

An oxidizing agent (accepts/donates) hydrogen/electrons

A

accepts e-

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12
Q

In respiration glucose is the (oxidizing/reducing) agent

A

reducing agent

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13
Q

In respiration 6O₂ is the (oxidizing/reducing) agent

A

oxidizing agent

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14
Q

NAD⁺ (4 facts)

A
  1. is an electron carrier 2. has one positive charge 3. can carry 2 electrons and 1 proton 4. transfers electrons gained in the oxidation of glucose to drive ATP synthesis
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15
Q

NADH

A

transfers electrons at a high energy level, creates ~ 3 ATP per NADH

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16
Q

NAD⁺ is a(n) ____ agent

A

oxidizing agent (accepts e-)

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17
Q

NADH is a(n) ____ agent

A

reducing agent (donates e-)

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18
Q

FAD transfers electron from ___ to the electron transport system

A

citric acid cycle

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19
Q

FADH₂ transports at a ____ energy level than NADH, and makes ~ ____ ATP per FADH₂

A

lower, 2

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20
Q

substrate level phosphorylation creates ATP by ____

A

transfering a phosphate from a high-energy phosphate compound to ADP by kinases

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21
Q

when does substrate level phosphorylation occur and where

A

during glycolysis in the cytoplasm

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22
Q

substrate level phosphorylation is (aerobic/anaerobic)

A

anaerobic fermentation

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23
Q

Where does oxidative phosphorylation occur?

A

In the inner and intermembrane space of the mitochondria in eukaryotic cells. In prokaryotic cells it occurs in the plasma membrane.

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24
Q

Chemiosmotic theory (by Peter Mitchell)

A

NADH or FADH₂ transfers electrons taken from food molecules and through the electron transport chain to be accepted by O₂.

25
Electron transport chain (def)
A series of membrane proteins
26
Oxidative phosphorylation
NADH or FADH₂ transport electrons taken from food molecules through the electrons transport chain and accepted by O₂. (H⁺ are pumped into the intermembrane space of the mitochondria from the matrix creating a [H⁺] gradient.)
27
Membrane protein (def)
a proton pump that creates a proton gradient
28
H⁺ gradient
proton gradient formed from differences in proton concentrations between the inside and outside of a membrane.
29
ATP synthase
a membrane protein complex that creates ATP by combining ADP with Pi. When the H⁺s return to the matrix through ATP synthase it releases an ATP.
30
Pi
inorganic phosphate
31
Proton motive force
H⁺s returning to the matrix because of their concentration gradient.
32
glycolysis (simple def)
the breakdown of sugar
33
glycolysis (short process)
- occurs in the cytoplasm - 2 stages - first stage is energy investing (2 ATP are used to break down glycolysis into 2 two trioses) - second stage is where 4 ATP, 2 pyruates, and 2 NADH are formed
34
Hexokinase
phosphorylation enzyme that phosphorylates glucose (step 1 of glycolysis)
35
Phosphofructokinase
kinase that phosphorylates a phospho-fructose. - complex allosteric enzyme - enzyme that needs Mg ²⁺ - phosphorylyses fructose 6-phosphate (step 3 of glycolysis) - can be inhibited by citrate?
36
What is the key regulatory step of glycolysis?
- step 3 - phosphorylation of fructose 6-phosphate - also uses ATP like step 1
37
What is the key regulatory enzyme of glycolysis?
Phosphofructokinase - because it can make the reaction go faster, slower, or stop
38
What is the first commited step of glycolysis?
Step 1: glucose is phosphorylated by hexokinase into glucose 6-phosphate
39
Arsenate poisoning
Competes with PO4^2- for -SH site, creates inhibition so no ATP is made
40
What are the imputs of glycolysis (4)?
- glucose - 2 ATP - 2 NAD⁺ - 2 ADP - 2 Pi
41
What are the outputs of glycolysis (4)?
- 2 pyruvate - 2 NADH - 2 H⁺ - 2 ATP (net)
42
Glycerol 3-phosphate shuttle
NADH from cytoplasm transfers electron to DHAP to form glycerol 3-phosphate that enters mitochondria to form FADH2 and DHAP from FAD. - only makes 2 ATP per NADH
43
Malate-Aspertate shuttle
In heart and liver cells, NADH transfers electron to mitochondria NADH through malate-aspertate, making 3 ATP per NADH per glycolysis
44
Acetyl CoA formation
Pyruvate from glycolysis is transported to the mitochondria to be oxidized by the pyruvate dehydrogenase complex into acetyl CoA - can also be generated through fats and oxidative phosphorylation - irreversible and highly regulated process - Acetyl CoA is a common intermediate that can be gotten from carbohydrates, lipids, and proteins
45
Acetyl CoA reactants and products
R: pyruvate, CoA, NAD+ P: Acetyl CoA, CO2, NADH
46
What is the first commited step of the Krebs Cycle?
- first step - performed by citrate synthase - induced by AMP - inhibited by ATP
47
Krebs Cycle first commited step reactants and products?
R: Acetyl CoA, Oxaloacetate, H2O P: Citrate, CoA, H
48
Krebs Cycle step 3
- rate limiting step - Oxidative dicarboxylation of isocitrate by isocitrate dehydrogenase - stimulated by ADP - inhibited by high levels of NADH and ATP
49
Krebs Cycle step 3 reactants and products?
R: Isocitrate, NAD+, H+ P: Ketoglutarate, NADH+, CO2, H+
50
Krebs Cycle reactions inputs
Acetyl CoA, 3NAD+, FAD, ADP, Pi, 2H2O
51
Krebs Cycle reactions outputs
2CO2, 3NADH, 3H+, FADH, ATP, CoA
52
ATP/ADP Translocase
A proton transports the ATP released from the matrix to the cytoplasm through the membrane protein ATP/ADP translocase
53
What are the three types of respiratory poisons?
1.) Uncouplers of proton gradient 2.) ATP synthase inhibitors 3.) Electron transport inhibitors
54
Uncouplers of proton gradient (def)
get rid of proton gradient by making the membrane leaky, stops ATP from being made
55
ATP synthase inhibitors (def)
binds to ATP synthase and inhibits ATP synthesis
56
Electron transport inhibitors (def)
blocks the electron transport causes reduced or lack of protein gradient stops the regeneration of NAD+ and FAD so Krebs cycle cannot function
57
Alcohol fermentation
2 pyruvates (from glycolysis) are decarboxylated to make acetaldehyde, which is hydrogenated to form ethanol using NADH as the reducing agent. This regenerates NAD+ allowing glycolysis to continue
58
Pasteur effect
yeast growing under anaerobic conditions consume more sugar than yeast growing under aerobic conditions this is because yeast growing under anaerobic conditions also produce less ATP
59
Lactic acid fermentation
animals do not have pyruvate decarboxylase so they do this instead. NAD+ is regenerated by converting the pyruvate into lactate to be taken to the liver to be converted to glucose for later use