Results

Question 1

What did we expect for MIC for bleMBL and what happened?

Answer 1

We expected 16 in 0,4 and got the opposite

Question 2

What does the copy number have to say?

Answer 2

It has a lot to say for stability because of the division, if there is a high copy number the probability is high for every cell in the divison to get a copy.to ensure that every cell gets the plasmid has its systems

Question 3

what does the selection coefficient say?

Answer 3

represent the change in resistance over time
a positive selection coefficients means resistance will increase in prevalence over time. MSC itself is the concentration where the selection coefficient = 0. resistance will neither increase nor decrease over time.

Question 4

how do we calculate the selection coefficients?

Answer 4

first by calculating the ratios from the resistant/susceptible. the first graph was calculated by taking mean of the ratios from the replicates. then s(0-t48) was calculated from the generations. the coefficient was then calculated by taking the means of the s(0-t48)

Question 5

Why did the MIC strips for imipenem look so weird?

Answer 5

overexpression by both promoters and costly for them bith and thats why the growth is not confluent

Question 6

what is compensatory evolution

Answer 6

a hypothesis that even if a plasmid produce a cost when first introduced to a host the cost, could be alleviated over time through compensatory mutations in the plasmid or the host chromosome.