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mlsci 250 > reticulin stain > Flashcards

reticulin stain Flashcards

1
Q

what collagen type makes up reticulin

A

type III collagen

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1
Q

what is reticulin

A

supporting mesh or organs and tissues, particularly liver, lymphatic system, bone marrow

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2
Q

reticulin is a sensitive indicator of

A

organ damage
- staining is used extensively for liver pathology

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3
Q

what is the principle of reticulin silver staining

A
  • metallic impregnation
  • silver reacts with active groups on reticulin
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4
Q

steps of reticulin staining

A
  1. Oxidation (Potassium Permanganate)
  2. Bleaching (Oxalic Acid)
  3. Sensitization (Ferric Ammonium Sulphate)
  4. Silver Impregnation (ammoniacal silver)
  5. Reducing (Formalin)
  6. Toning (Gold Chloride)
  7. Rinsing (Sodium Thiosulphate)
  8. Counterstaining (Eosin)
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5
Q

what are some precautions of reticulin staining

A
  • amoniacal silver is preapres fresh and very sensitive to pH
  • sensitization agen is tenuous and should not be subject to aggressive washing
  • unreduced silver left on slide will darken with time
  • high basic solutions may cause tissue to lift off slide
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mlsci 250 (11 decks)

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