review Flashcards
(141 cards)
Chromosomes condense. The spindle apparatus
begins to form, and polar microtubules overlap each other
Prophase
In cells of many organisms, the nuclear envelope
disintegrates. Microtubules attach to the kinetochores
of chromosomes and begin moving them to the middle of the
spindle.
Prometaphase
All the chromosomes are positioned in the middle
of the spindle. The spindle is anchored to the cell membrane
by astral microtubules.
Metaphase
Sister chromatids are pulled apart by the disassembly
of kinetochore microtubules at the kinetochore. The
separated chromatids are now daughter chromosomes. The
spindle poles are moved farther apart to fully separate the replicated
chromosomes.
Anaphase
Telophase
Daughter chromosomes are fully separated and
are clustered at opposite poles of the spindle. A nuclear envelope
forms around each set and the chromosomes de-condense.
How Do Cells Replicate?
when a cell divides it must copy its chromosomes, separate the copies, and divide the cytoplasm to generate daughter cells such that each carries the same chromosomal complement as the parent.
interphase consists of s phase
is when chromosomes are replicated and gap phases called g1 and g2 when cells grow and prepare for division
eukaryotic cells divide by cycling through four phases
g1, s , g2, and M and enter a non replication phase called g0
eukaryotic cells divide by
alternating between interphase and M phase.
Which statement about the daughter cells following mitosis and cytokinesis is correct?
They are genetically identical with each other and with the
parent cell
Progression through the cell cycle is regulated by oscillations in
the concentration of which type of molecule?
cyclins
After the S phase, what comprises a single chromosome
two sister chromatids
What major events occur during anaphase of mitosis
Sister chromatids separate, and the spindle poles are pushed farther apart.
What evidence suggests that during anaphase, kinetochore
microtubules shorten at the kinetochore
Daughter chromosomes were observed to
move toward the pole faster than do the marked regions
of fluorescently labeled kinetochore microtubules.
Under normal conditions, what happens to the cell cycle if the chromosomes fail to separate properly at anaphase?
The cycle would arrest in M phase, and cytokinesis
would not occur.
Identify at least two events in the cell cycle that must be completed successfully for daughter cells to share an identical complement of chromosomes.
For daughter cells to have identical complements
of chromosomes, all the chromosomes must be
replicated during the S phase, the spindle apparatus
must connect with the kinetochores of each sister
chromatid in prometaphase, and the sister chromatids
of each replicated chromosome must be partitioned
in anaphase and fully separated into daughter cells
by cytokinesi
Explain how microinjection experiments supported the hypothesis
that specific molecules in the cytoplasm are involved in the
transition from interphase to M phase. What was the control for
this experiment?
Microinjection
experiments suggested that something in the cytoplasm
of M-phase cells activated the transition from
interphase to M phase. The control for this experiment
was to inject cytoplasm from a G2-arrested oocyte into
another G2-arrested oocyte
Why are most protein kinases considered regulatory proteins?
Protein kinases
phosphorylate proteins. Phosphorylation changes a
protein’s shape, altering its function (activating or
inactivating it). As a result, protein kinases regulate the
function of proteins.
Why are cyclins called cyclins? Explain their relationship to MPF activity
Cyclin concentrations
change during the cell cycle. At high concentration,
cyclins bind to a specific cyclin-dependent kinase (or
Cdk), forming a dimer. This dimer becomes active
MPF by changing its shape through the phosphorylation
(activating site) and dephosphorylation (inhibitory
site) of Cdk
A particular cell spends 4 hours in G1 phase, 2 hours
in S phase, 2 hours in G2 phase, and 30 minutes in M phase. If a
pulse–chase assay were performed with radioactive thymidine on
an asynchronous culture, what percentage of mitotic cells would
be radiolabeled after 9 hours?
adding up each phase allows you to determine
that the cell cycle is 8.5 hours long. After 9 hours,
the radiolabeled cells would have passed through a full
cycle and be in either S phase or G2—none would have
entered M phase
When fruit fly embryos first begin to develop, a large cell is
generated that contains over 8000 nuclei that are genetically identical with one another. What is most likely responsible for this
result?
The embryo passes through
multiple rounds of the cell cycle, but cytokinesis does not occur during M phases
What is most likely responsible for the reduction in death rates
over the past several years in cancers of the breast and prostate?
How is this related to the development of cancer?
Early detection of
cancers leads to a greater likelihood of survival. The
widespread implementation of breast and prostate exams
allows for the identification and removal of benign
tumors before they become malignant.
Cancer is primarily a disease of older people. Further, a group
of individuals may share a genetic predisposition to developing
certain types of cancer, yet vary a great deal in time of onset—or
not get the disease at all. What conclusion could be drawn based
on these observations? How does this relate to the requirements
for a cell to become cancerous?
Cancer
requires many defects. Older cells have had more time
to accumulate defects. Individuals with a genetic predisposition
to cancer start out with some cancer-related defects,
but this does not mean that the additional defects
required for cancer to occur will develop
In multicellular organisms, nondividing cells stay in G0 phase. For the
cell, why is it better to be held in G1 rather than S, G2, or M phase
G1 cells have not replicated their DNA in preparation for
division.