Rhabdoviridae

Question 1

How can one get Rhabdoviridae

Answer 1

aerosol droplets from bats

bite

Question 2

where does Rhabdoviridae multiply in the body

Answer 2

neural and extraneural tissues

Question 3

what is the genetic material for Rhabdoviridae

Answer 3

single stranded
non-segmented
negative polarity RNA

Question 4

Rhabdoviridae virus has to carry what with it in its genome

Answer 4

RNA dependent- RNA polymerase = L + NS protein

Question 5

what type of capsid does Rhabdoviridae have

Answer 5

helical nucleocapsid

Question 6

what does Rhabdoviridae look like under the microscope

Answer 6

bullet-shaped

Question 7

what is located on the Rhabdoviridae envelope and what does it due

Answer 7

glycoprotein G

• produces neutralizing Ab
• acts as a hemagglutinin
• inhibits cell processes
• target for producing vaccine

Question 8

what are the 2 cell receptors for Rhabdoviridae

Answer 8

1. nicotinic acetylcholine receptor

2. neural cell adhesion molecule

Question 9

genome replication and transcription occurs where in the cell for rabies

Answer 9

cytoplasm

Question 10

what are two events that must occur relative to the viral genomic RNA

Answer 10

transcription

genome replication

Question 11

what makes Rhabdoviridae virus a bullet shape

Answer 11

viral progeny buds form a site of plasma membrane

- has G glycoprotein extending from it’s envelope on its outer surface

Question 12

what are the 3 phases of rabies virus

Answer 12

incubation phase
prodromal phase
neurological phase

Question 13

how long is incubation phase

Answer 13

prolonged to weeks to months

Question 14

what are clinical symptoms for prodromal phase? what is happening during this phase

Answer 14

fever, malaise, headache, sore throat

viral transport called retrograde Axoplasmic

Question 15

in prodromal phase of rabies where does the virus infect, replicate and travel to

Answer 15

infectes peripheral nerves
replicates in dorsal ganglia
travels up spinal cord to brain

Question 16

what are the 2 stages in neurological phase

Answer 16

excitement stage

manic stage

Question 17

what are clinical symptoms for neurological phase

Answer 17

infection of brain

descend to infect eye, glands and visceral organs

Question 18

clinical symptoms for excitement stage

Answer 18

apprehension, fright, hydrophobia, fear of space- aerophobia

Question 19

clinical symptoms for manic stage

Answer 19

convulsions, usually death

Question 20

what is the number 1 source of rabies in US

Answer 20

bats

Question 21

what is the number 1 source of rabies in the world

Answer 21

dogs

Question 22

where is the virus found in the animal

Answer 22

saliva, CNS, urine, lymph, milk, blood

Question 23

where is the virus during the incubation stage

Answer 23

multiply in muscle or connective tissue at the bite site and begins to move along the peripheral nerves

Question 24

what does rabies destroy in the CNS

Answer 24

posterior horn

Question 25

what virus destroys the anterior horn

Answer 25

polio

Question 26

when do antibodies arise in the rabies infection

Answer 26

late in course, too late

Question 27

what are 2 main ways a doctor can diagnose rabies

Answer 27

Negri bodies in cytoplasmic inclusion from brainstem of infected human - detect virus from saliva, serum or corneal impression, skin and brain biopsies - - RT-PCR - -direct immunofluoresence test of animal tissue

Question 28

what is the gold standard diagnosis for rabiies

Answer 28

direct immunofluoresence test

Question 29

when can the vaccine been given to a person who already has rabiess

Answer 29

during incubation period

Question 30

what is semple

Answer 30

rabiesvirus infected nervous tissue vaccine - inactivated with phenol - 7-14 daily injections - evokes allergic ecephalomyelitis complications

Question 31

what is another vaccine for rabies? how many doses? | who usually gets it and why?

Answer 31

human diploid cell vaccine 5 doses prophylactic for animal handlers IM

Question 32

how is Human diploid cell vaccine administered in adults and children, where should it not be given

Answer 32

adults: deltoid children: anterolateral aspect of thigh or deltoid not in gluteal muscle: too much fat not enough muscle

Question 33

what absorbs the rabies vaccine absorbed

Answer 33

aluminum phosphate

Question 34

what is the less expensive vaccine for rabies

Answer 34

Chick Embryo vaccine

Question 35

what is the treatment of human bitten by rapid animal or expected rabid animal

Answer 35

1. flush wound with soap and water 2. day 0 inject 1/2 human rabies immune globulin directly into wound and inject 1/2 HRIG IM 3. Day 0 give vaccine but not at site of bite. 5 doses

Question 36

what viruses fall under the Filovirdiae family

Answer 36

Marbug virus | Ebola

Question 37

clinical symptoms for Marburg virus

Answer 37

African hemorrhagic fever liver encephalitis

Question 38

Marbug is found in what animal, how is it derived, and who usually gets infected with it

Answer 38

monkey tissue culture cells laboratory workers

Question 39

what are some clinical features of Marbug and ebola

Answer 39

fever, abdominal pain conjuctivitis rash, diarrhea

Question 40

what is the route of transmission for ebola

Answer 40

person to person