RNA Transcription: Flashcards
(20 cards)
What is Transcription?
It’s the synthesis of RNA using information in the DNA
-2 Nucleic Acids are wirtten in different forms of the same language, and the information is simply transcribed/ REWRITTEN from DNA to RNA
Messenger RNA (mRNA):
- A type of RNA molecule
- It carries a genetic message from the DNA to the protein-synthesizing machinery of the cell..
Transcription and Translation occurs:
In all organisms
-both bacteria and archea and eukaryotes as well
Transcription occurs in the _________, and mRNA is then transported to the __________, where translation occurs.
- Nucleus
- Cytoplasm
Translation is:
-the synthesis of a polypeptide using the information in the mRNA.
During Translation the cell must:
-Translate the nucleotide sequence of an mRNA molecule into the amino acid sequence of a polypeptide.
The Site of translation are ___________.
Ribosomes which are complex particles that facillitate the orderly linking of amino acids into polypeptide chains.
The transcription of a protein-coding gene results in ________, and further processing yields the finished ________.
- pre-mRNA
- mRNA
The series of words in a gene is transcribed into a a complementary series of _______________, which is then translated into a _____________.
- non-overlapping, three nucleotide words
- chain of amino acids
Introns are removed from pre-mRNA by:
RNA splicing
The 3 stages of Transcription:
1-Initiation’
2-Elongation
3-termination of the RNA Chains
What is a transcription start point?
-The nucleotide where RNA synthesis actually begins
The mRNA molecule is ___________ to its DNA template because__________.
- Complementary
- RNA nucleotides (which contain Ribose) are assembled on the template according to base-pairing rules
- Also used for the DNA nucleotide triplets along the nontemplate strand.
The mRNA nucleotide triplets are called_________ and are customarily written in the ____________.
Codons
5’ to 3’
DNA nucleotide triplets along the nontemplate strand, codons are ________ to the template strand and _______ to the mRNA (except T instead of U).
- complementary
- Identical
During translation the sequence of codons along an mRNA molecule is:
decoded, or translated, into a sequence of amino acids making up a polypeptide chain
Unlike DNA polymerases, RNA polymerases are able to:
start a chain from scratch; they don’t need a primer
Molecular components of Transcription:
-Enzyme RNA polymerase pries the 2 strand of DNA apart & joins together RNA nucleotides complementary to the DNA template strand, elongating the RNA polynucleotide. Assembling in the 5’ to 3’ direction. The RNA polymerase attaches to the promoter, initiation transcription.
The direction of transcription is
“downstream”
RNA Polymerase II:
used for mRNA synthesis