Role of microbiology lab

Question 1

what are the 2 key functions of a microbiology lab

Answer 1

clinical - diagnosis of infections for everyday management of patients
epidemiological - support for infection prevention and control in searching for source and route of transmission

Question 2

name 3 requirements for microbiological services

Answer 2

• available everyday, ideally 24 hours
• able to examine different types of biological specimens e.g blood, saliva
• identify common bacteria and fungi to species level

Question 3

staphyl v strept (ococci)

Answer 3

staphyl - clusters
strept - chains

Question 4

what is the theory of the gram stain

Answer 4

gram positive bacteria have a thick layer of peptidoglycan in their walls allowing them to retain the gram stain
gram negative cells do not and do not retain the stain

Question 5

antibiotic stewardship

Answer 5

controlling use of antibiotics
provide leadership to antimicrobial team, do antibiotic ward rounds, active awareness and surveillance

Question 6

chain of infection model and example of how broken

Answer 6

infectious agent - diagnosis and tx
reservoir - disinfection, environmental sanitation
portal of exit - handwashing, control of aerosols
mode of transmission - handwashing, isolation
portal of entry - personal hygiene, hand washing
susceptible host - immunisation, treatment of underlying diseases

Question 7

what method of culture might you use for pseudomembranous candidiasis

Answer 7

smear

Question 8

what method of culture might you use for denture stomatitis

Answer 8

oral rinse

Question 9

what method of culture might you use for angular chellitis

Answer 9

swab

Question 10

what method of culture might you use for a periodontal abscess

Answer 10

needle aspirate

Question 11

name 3 virulence factors of candida

Answer 11

phospholipase - aids host cell penetration
haemolysin - degrades RBCs
proteinase - degrades proteins and aids adhesion