RPAs Flashcards

(103 cards)

1
Q

Field investigation RPA:

What is the purpose of the RPA?

A

investigating a species population size and distribution

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2
Q

Field investigation RPA:

How do find the area of the habitat?

A

Base x height

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3
Q

Field investigation RPA:

What do you do with the Quadrat?

A

Throw randomly onto the habitat

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4
Q

Field investigation RPA:

What do you do once you’ve thrown the Quadrat? 2pts

A

Count the amount of species in the Quadrat and record the number

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5
Q

Field investigation RPA:

How many times do you throw the Quadrat?

A

10 times

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6
Q

Field investigation RPA:

what do you do with the results table?

A

Find the mean number of species per Quadrat

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7
Q

Field investigation RPA:

How do you estimate the population size? 2pts

A

Divide the habitat area by area of 1 Quadrat

Then multiply that by the mean number

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8
Q

Field investigation (transect) RPA:

What do you do to make the transect line?

A

A measuring tape

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9
Q

Field investigation (transect) RPA:

what do you do once you have drawn the transect?

A

Place a Quadrat at equal intervals along the transect line

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10
Q

Field investigation (transect) RPA:

what do you do to each Quadrat along the transect?

A

Use a light meter to test the light intensity of each Quadrat

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11
Q

Field investigation (transect) RPA:

what do you do with the recorded results?

A

Analyse the correlation between light intensity and population of each Quadrat

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12
Q

Germination RPA:

what is the purpose of this RPA?

A

To investigate the factor of light on seedling growth

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13
Q

Germination RPA:

What is phototropism?

A

The ability of plants to grow away or towards light

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14
Q

Germination RPA:

How many Petri dishes do you use?

A

3

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15
Q

Germination RPA:

What do you fill the Petri dishes with?

A

Damp cotton wool and 10 mustard seeds

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16
Q

Germination RPA:

What do you do to the dishes once seeds have been placed?

A

Leave in the warm to germinate

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17
Q

Germination RPA:

What do you do with the dishes once they have germinated? 3pts

A

Place one in direct sunlight

One in partial sunlight

One in complete darkness

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18
Q

Germination RPA:

How long do you leave the seedlings?

A

5 days

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19
Q

Germination RPA:

How often do you check the seedlings? What do you do when you check them?

A

Every day and measure the height

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20
Q

Germination RPA:

What do you do with the results?

A

Calculate the mean height of each seedling everyday of each dish

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21
Q

Germination RPA:

Which one would the tallest be?

A

One in darkness

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22
Q

Germination RPA:

which one would be yellowing? Why?

A

One in darkness
No sunlight which uncoloured the chlorophyll

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23
Q

Photosynthesis RPA:

What is the purpose of this investigation?

A

Investigating the effect of light intensity on photosynthesis

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24
Q

Photosynthesis RPA:

Where do you place the lamp throughout the investigation? 2pts

A

Start 5cm away from pondweed
Move 5cm away every 30 seconds

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25
Photosynthesis RPA: What are you counting every 30 seconds?
Number of bubbles coming out the pondweed tip
26
Photosynthesis RPA: What is the boiling tube filled with? 2pts
Cut pondweed Water
27
Photosynthesis RPA: What does a large number of bubbles mean?
Fast rate of photosynthesis
28
Photosynthesis RPA: What happens to the number of bubbles the further you move the light away?
Rate of photosynthesis decrease, amount of bubbles decreases
29
Photosynthesis RPA: How many times do you move the light?
10 times
30
Photosynthesis RPA: What do you do with the results?
Plot a graph
31
Microbiology RPA: What is the purpose of this investigation?
Investigating the effect of antiseptics on bacterial growth
32
Microbiology RPA: What do you do first thing to the agar plate?
Treat it with bacteria
33
Microbiology RPA: How many antiseptics do you use? What else do you use?
3 antiseptics 1 control (nothing)
34
Microbiology RPA: Why do we use a control?
So we can see how the antiseptics compare
35
Microbiology RPA: How do you divide the Agar Plate?
Equally into four sections by marking it with a cross
36
Microbiology RPA: What do you use the forceps for?
To place the antiseptic filter paper disk onto each section (apart from the control)
37
Microbiology RPA: What do you do to the control?
Place a blank filter disk onto the section
38
Microbiology RPA: How do you stop any unwanted bacterial growth?
Secure the agar plate with transparent tape (not fully though)
39
Microbiology RPA: After how long do you measure the ZOI (zone of inhibition)
One week later
40
Microbiology RPA: How do you measure the Area of inhibition?
Pi times radius squared
41
Germination RPA: At what temperature do we leave the agar plate at? Why?
25°C To prevent dangerous bacterial growth
42
Enzyme RPA: What is the purpose of this investigation?
To investigate the activity of amylase at different pHs
43
Enzyme RPA: What does amylase do?
Breaks down starch into simple sugars
44
Enzyme RPA: What colour does iodine turn in the presence of starch?
Blue-black
45
Enzyme RPA: How much starch do you measure?
5cm3
46
Enzyme RPA: How much buffer solution do you measure out? How many test tubes do you put it in?
2cm3 2 test tubes
47
Enzyme RPA: What do you put in both test tubes?
5cm3 of starch 2cm3 of buffer solution
48
Enzyme RPA: What do you do differently to the test tubes?
Add 5cm3 of amylase to one Add 5cm3 of distilled water to the other
49
Enzyme RPA: Where do you place both test tubes once everything has been added?
Place both in water bath for 30 seconds
50
Enzyme RPA: What do you do to the test tubes once they have been left in water baths for 30 seconds? 2pts
Use 2 pipettes of each solution onto the spotting tile Add a drop of iodine to each one
51
Enzyme RPA: How many times do you add the two solutions to the tile?
Until there is no colour change
52
Enzyme RPA: Why does the temperature have to be so specific?
So the amylase doesn’t denature
53
Enzyme RPA: What does the test tube with distilled water act as? What will it’s results always be?
The control Will always turn Blue-Black
54
Microscopy RPA: What is the purpose of this investigation?
To view sub cellular structures close up of both animal and plant cells
55
Microscopy RPA: What cells are used? What has to happen to both?
Human cheek cells Onion cells Stained with iodine
56
Microscopy RPA: What equation is used in this investigation?
Image size = actual size x magnification
57
Microscopy RPA: What unit is actual size measured in?
Micrometers (um)
58
Microscopy RPA: What type of microscope is used?
Light microscope
59
Microscopy RPA: What do we use the coarse adjustment knob for?
To move the stage up and down
60
Microscopy RPA: What magnification do we position it at?
X40
61
Microscopy RPA: What do we look through when observing the cells
The eyepiece
62
Microscopy RPA: What do you use the fine adjustment knob for?
To bring the image into focus
63
Microscopy RPA: What do you put the cells on? 3pts
On the slide Put iodine on Put glass cover slip over the top
64
Osmosis RPA: What is the purpose of this investigation?
To investigate osmosis using sugar solutions and plant tissue
65
Osmosis RPA: What is osmosis?
The movement of water from an area of low solute to an area of higher solute across a PPM
66
Osmosis RPA: How do you cut up the potato tissue? 2pts
Cut 5 equal pieces of potato using a cylindrical cutter Weigh their mass
67
Osmosis RPA: What do you do with the solutions?
Measure the same volume of different sugar concentrations + 1 distilled water solution Start with 0.25mol/dm3 of sugar solution and go up Measure 4 sugar solutions and 1 distilled water solution
68
Osmosis RPA: How long do you leave the tissue in the boiling tubes of solution for?
30 minutes
69
Osmosis RPA: What do you do once you’ve taken the tissue out the solution?
Record the new mass
70
Osmosis RPA: How do you calculate the percentage change in mass?
Change ————- x 100 Original
71
Osmosis RPA: What is on the X-Axis when plotting the graph?
Concentration
72
Osmosis RPA: What does an increase in mass mean?
More water has moved into the tissue because the concentration was lower in the solution
73
Reaction time RPA: What is the purpose of this investigation?
To investigate the effect of practice on reaction time
74
Reaction time RPA: How many people are used in this investigation?
Two
75
Reaction time RPA: How is the ruler used in this investigation? 2pts
One person will catch it between their finger and thumb It will be level with their finger at 0cm
76
Reaction time RPA: Does the person catching the ruler know when it’s going to be dropped?
No
77
Reaction time PRA: What must the student remember to do to the results of the ruler?
Repeat 10 times
78
Reaction time RPA: What two variables are measured in this RPA:
Reaction time The cm they caught the ruler at
79
Reaction time RPA: What happens to the reaction time the more people practice?
It decreases
80
Reaction time RPA: What factors effect reaction time?
Caffeine Tiredness Age
81
Food tests RPA: What chemicals is used to test for lipids?
Ethanol + water
82
Food tests RPA: What colour will the substance turn if lipids is present?
Milky white
83
Food tests RPA: What colour will the substance turn if starch is present?
Blue-black
84
Food tests RPA: What chemical is used to test for starch?
Iodine
85
Food tests RPA: What chemical is used to test for sugars
Benedict’s solution
86
Food tests RPA: What colour will the substance turn if sugar is present?
Orange-red
87
Food tests RPA: In what food test must the substance be heated with the chemical?
Glucose/sugar test
88
Food test RPA: What degree must the water bath be heated at for the glucose test?
80°c
89
Food tests RPA: What colour will the substance turn if protein is present?
Violet
90
Food tests RPA: What chemical is used to test for protein?
Biurets solution
91
Rate of decay RPA: What is the purpose of this RPA:
To investigate the effect of temperature on the rate of decay
92
Rate of decay RPA: How much milk do you add? And what do you put it in?
5cm3 Boiling tube
93
Rate of decay RPA: What else do you add to the boiling tube of milk? What do you add after that? What happens?
Cresol red indicator 7cm3 of sodium carbonate (should turn red/purple)
94
Rate of decay RPA: What digestive enzyme do you use? What do you do with it?
Lipase Add 5cm3 to another boiling tube
95
Rate of decay RPA: What do you do to both boiling tubes?
Place in water bath until they reach 30°c
96
Rate of decay RPA: What do you do to the boiling tubes after they have been heated? 2pts
Transfer 1cm3 of lipase solution to the milk boiling tube Record how long it takes for it to turn yellow
97
Rate of decay RPA: How many times do you repeat the lipase transfer at different temperatures?
Until the milk doesn’t turn yellow anymore
98
Rate of decay RPA: Why would the milk stop turning yellow eventually?
The lipase would have denatured and no longer would work
99
Rate of decay RPA: When drawing a graph of the results, what should be on the x-axis and y-axis?
Temperature Time
100
Rate of decay RPA: What does milk produce when it decays? What does it do?
Lactic acid Reduces the pH
101
Germination RPA: What does auxin control?
Controls growth
102
Germination RPA: What do plants use auxin to grow towards? 2pts
Gravity Light
103
Germination RPA: What is tropism?
The ability for a plant to move in response to a stimulus (light + gravity)