Sample Collection, Handling, Preservation, and Transport Flashcards

(50 cards)

1
Q

Most commonly submitted sample for parasite examinations

A

STOOL

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2
Q

The most common procedure performed in area of parasitology

A

Examination of a stool specimen for ova and parasites (O&P)

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3
Q

test for ova fecalysis is always ______

A

stool sample

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4
Q

Fecal specimens should be collected in

A

clean, wide mouthed/watertight containers with tight-fitting
lids, waxed cardboard box (1/2 pint) or matchbox.

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5
Q

_______ should not be allowed to contaminate the stool specimen

A

Urine

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6
Q

Container used on basic fecalysis

A

Clean Container

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7
Q

Container used on stool culture

A

Sterile Container

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8
Q

Stool ___ be retrieved from toilet bowl water
because

A

Should not

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9
Q

The typical stool collection protocol consists of _
specimens.

A

3

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10
Q

one specimen collected every other day or a total of _ collected in _ days

A

3;10

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11
Q

Other name for protozoan infection (severe diarrhea)

A

Amebiasis

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12
Q

Stool samples from patients whose therapy includes barium, bismuth or mineral oil should be collected

A

prior to therapy or not until 5-7 days

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13
Q

What element is taken for diarrhea

A

Bismuth

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14
Q

Collection of specimens from patients who have taken antibiotics or antimalarial medications should be delayed for _ weeks

A

2

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15
Q

Patients who have been treated for protozoan infections are typically checked __ weeks after therapy.

A

3-4

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16
Q

A patient treated for helminth infection may be checked ___ weeks post therapy

A

1-2

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17
Q

Taenia may be delayed for ___ weeks post therapy

A

5-6

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18
Q

Routine stool examination requires a ____ sized/marble stool

A

Thumb

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19
Q

formed stool or about ___
tablespoons (__ mL) of watery/diarrheic stool.

A

5-6;10

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20
Q

Liquid specimens be examined within ___

A

30 minutes

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21
Q

semi- formed specimens should be examined within ___ of passage

22
Q

If a number of specimens are received at the same time, pick out the_____ stools

A

liquid;watery

23
Q

____ in soft or watery stools may possibly yield the presence of trophozoites

A

Blood and mucus

24
Q

By gross examination, tapeworm ____ and
adult nematodes may be found on the stool sample

25
Recovery of the ____ on the stool sample following therapy indicates success of treatment.
tapeworm scolex
26
Feeding stage of protozoa
TROPHOZOITES
27
Diagnostic stage of protozoa
TROPHOZOITES
28
Non-feeding stage of protozoa
CYSTS
29
Infective stage of protozoa
CYSTS
30
A ___ collected stool sample, which is immediately submitted to the laboratory, is the ideal specimen for parasitic examination
freshly
31
Are substances that preserve the morphology of protozoa and prevent further development of certain helminth eggs and larvae
Fixatives
32
The ratio of fixative to stool
3:1
33
The specimen must be fixed in the preservative for at least ___ before processing begins
30 minutes
34
T or F: Temporary storage of fecal samples in a refrigerator (4-8°C) may be acceptable.
T
35
T or F: Never freeze stool samples nor keep them in incubators
T
36
Refrigerated samples are only suitable for ___testing (CDC, 2016)
antigen
37
An aqueous solution of formaldehyde, about 37-40% weight in volume.
FORMALIN
38
Recommended concentration of formalin for preservation of protozoan cyst
>5%
39
Recommended concentration of formalin for preservation of helminth eggs and larvae
>10%
40
Comprised of a plastic powder that acts as an adhesive for the stool specimen when preparing slides for staining.
POLYVINYL ALCOHOL (PVA)
41
Most often combined with Schaudinn's solution, which usually contains zinc sulfate, copper sulfate or mercuric chloride as a base.
POLYVINYL ALCOHOL (PVA)
42
Ecofriendly fixative (PVA)
ECOFIX
43
Universal fixative, ecofriendly, contains no formalin, no PVA and no mercury.
TOTAL-FIX
44
A combination of preservative and stain for fecal specimens, especially used in field surveys.
MERTHIOLATE-IODINE-FORMALDEHYDE
45
For many years, considered as the "GOLD STANDARD"
SCHAUDINN’S SOLUTION
46
Alternate to Schaudinn's; contains 10% formalin as a fixative plus sodium acetate which acts as a buffer.
SODIUM-ACETATE FORMALDEHYDE
47
The specimen should be placed into a _____ for transport to the laboratory.
Zip lock plastic bag
48
10% Formalin (for wet mount)
1 part stool 3 parts formalin Place in vial, crush stool thoroughly, preserves specimen indefinitely if bottle is closed
49
MIF (for wet mount)
4.7 mL of MIF 0.3 mL of Lugol's lodine in a vial/bottle (just before dispatching) Add 2 mL of stool, crush with a glass rod/stick; preserves specimen indefinitely
50
PVA (for permanent staining)
30 mL of PVA or 3/4 full in a bottle/vial Enough fresh stool to fill the container Break stool with a stick and cover, preserves all forms of parasites indefinitely