SBL

Question 1

CLL/SLL

flow, stain

Answer 1

Flow: Kappa-restricted CD19, CD5 clone

Stain CD20, CD5 (No 19)

Question 2

Somatic Mutation BCL6

Answer 2

DLBL

Question 3

Hairy Cell Leukemia Markers

Answer 3

CD25 and CD11c

Poor Harry went bald at 25

Question 4

Plasma Cell Marker

Answer 4

CD138

Question 5

Reed Sternberg Cells Markers

Answer 5

CD15, CD30, Pax-5 Classic Hodgkin Lymphoma

B cell origin but has no CD20

Question 6

ALK +VE

Answer 6

Anaplastic Large Cell Lymphoma (ALCL)

doughnut/horseshoe cell 
young pple (love doughnuts) - better prognosis 
older - poor prognosis... they get faaaat with doughnuts !

Question 7

Langerhans Cell Histiocytosis Markers

Answer 7

CD1a and S100

EOSINOPHILS love it

Question 8

JAK2 Kinase

Answer 8

driver for Myoproliferative neoplasms

Question 9

early Lymphoblast marker for immaturity

Answer 9

TdT (can be proB or proT )

Question 10

CD34

Answer 10

early myeloid lineages

Question 11

Leukocyte common antigen

Answer 11

CD45

Question 12

Mature B cell MARKER

Answer 12

cd19, cd20, cd10, Pax-5

Question 13

cd16, cd56

Answer 13

NK cells

666 - killer number!!!!!

Question 14

mature T cell

Answer 14

CD1-CD8 (not 6/9)

Question 15

Burkitt Lymphoma translocation

effects
stain marker

Answer 15

t(8;14) -> increased MYC oncogene = proliferation
IgH/Myc

DJ Drop the Heavy Myc at 8:14pm when the Burkley starry sky comes out

Tingible macrophages clearing debris
Ki-67 proliferation marker on stain

Question 16

t(11;14)

Answer 16

cyclinD1/IgH
Mantle cell lymphoma –> in mucosa of stomach- lymphomatoid polyposis

11-14 y.old Mentors cycle!

Question 17

RUNX1-RUNX1T1 fusion

translocation
age grp with good prog

Answer 17

t (8;21) AML with neutrophilic maturation

Amil RUNs 821 miles around CBF Block - gets BLASTed and arrested

young pt - good prognosis

Question 18

APL (Acute Promyelocytic Leukemia)

effect and risk

Stain

Answer 18

t(15;17) PML-RARA
AML subtype

no RA binding -> no differentiation to PMN -> MORE BLASTS

DIC RISK (blasts upregulate TF -> hypercoagulation, increase annexin II receptors -> plasminogen -plasmin - fibrinolysis -> bleed

Stain: MPO+ve Auer Rods

Question 19

Immatunib

Answer 19

TKI - treats Philadelphia Chromosome
(T9:22) CML

BCR-ABL, -> prolif

Philly Cheese Man is ABL to love active TK

increased leukocytosis, big buffy coat, splenomegalu, EM Hematopoiesis

Question 20

Follicular Lymphoma

translocation , genes, effect

Answer 20

t(14,18) IgH/BCL-2 apoptosis suppressor

Grade 3 - more centroblasts

Question 21

ALL translocation

Answer 21

t(12, 21) - has better prognosis than without this translocation

Question 22

ALL Markers

tx

Answer 22

B cell markers: cd19, cd20, cd10, Pax-5
T cell: CD1-CD8 (not 6/9)
Tdt: in promylolympocytes (T and B)

Tx: chemo, intrathecal therapy

Question 23

Ritcher Transformation drivers

Answer 23

TP53/MYC

CLL/SLL transform to aggressive tumor

Question 24

Popcorn Cells, CD20 marker

Answer 24

Nodular LP Hodgkin Lymphoma (negative for CD15 AND cd30 whereas classic HL has them)

can transform to DLBCL like Follicular Lymphoma