Screening Test for Phagocytic Engulfment

Question 1

In the Phagocytic Engulfment Procedure, how many ml of Heparinized Blood in a Heparin Tube is needed?

Answer 1

2ml

Question 2

If a Heparin Blood Tube is not available, what is the substitute?

Answer 2

15-20 Heparinized Capillary Tubes

Question 3

What type of Tube Container is used for this experiment?

Answer 3

Heparin Tube

Question 4

What is the color of the Stopper in Heparin Tube?

Answer 4

Green

Question 5

How many test tubes are needed in the process?

Answer 5

2 test tubes

Question 6

The 2 test tubes needed for this experiment are
labeled:

Answer 6

Tube 1: Patient Test Tube
Tube 2: Control Test Tube

(In no particular order)

Question 7

How many drops of Buffer Coat is added from either the Patient’s Heparinized Blood or from the Normal Control to the respectively labeled tubes?

Answer 7

4 to 8 drops

Question 8

How many drops of Bacterial Broth Culture is added to the 2 test tubes (patient test tube and control test tube).

Answer 8

2 to 3 drops

Question 9

Materials needed for the Phagocytic Engulfment Procedure:

Answer 9

Broth Culture of Bacillus subtilis or Staphylococcus epidermidis
Microscope Slides
Pasteur Pipettes
Rubber Bulb
Test Tubes
Wright’s Stain

Question 10

In the incubation of both tubes, what is the preferred time and temperature?

Answer 10

37 degree Celsius for 30 minutes

Question 11

How many drops of the incubated specimen is placed in the glass slide and prepared for smear viewing?

Answer 11

1 drop

Question 12

Wright’s Stain Procedure:

Answer 12

1. Cover each smear generously with filtered Wright’s Stain and allow the stain to remain in the slide for 5 minutes.
2. Slowly add distilled water or buffer to the stain until the buffer begins to overflow the stain. Watch for the appearance of a metallic luster.
3. Gently blow on the slide to mix the stain and buffer.
4. Allow the buffer to remain on the slide for at least 5 minutes.
5. Gently wash the stain and buffer off the slide with distilled water.
6. Air dry or carefully blot the slide between two sheets of bibulous paper.

Question 13

How many minutes is needed to allow the Wright’s Stain to remain on the slide?

Answer 13

5 Minutes

Question 14

What appearance will show after we add distilled water or buffer into the stain?

Answer 14

Metallic luster appearance

Question 15

After the staining process, we will now examine the smear under what objective?

Answer 15

Oil Immersion Objective (100x)

Question 16

Identify what Step Number in the Wright’s Staining Process:

Cover each smear generously with filtered Wright’s Stain and allow the stain to remain in the slide for 5 minutes.

Answer 16

Step 1

Question 17

Identify what Step Number in the Wright’s Staining Procedure:

Slowly add distilled water or buffer to the stain until the buffer begins to overflow the stain. Watch for the appearance of a metallic luster.

Answer 17

2

Question 18

Identify what Step Number in the Wright’s Staining Procedure:

Gently blow on the slide to mix the stain and buffer.

Answer 18

Step 3

Question 19

Identify what Step Number in the Wright’s Staining Procedure:

Allow the buffer to remain on the slide for at least 5 minutes.

Answer 19

Step 4

Question 20

Identify what Step Number in the Wright’s Staining Procedure:

Gently wash the stain and buffer off the slide with distilled water.

Answer 20

Step 5

Question 21

Identify what Step Number in the Wright’s Staining Procedure:

Air dry or carefully blot the slide between two sheets of bibulous paper.

Answer 21

Step 6

Question 22

Positive results reveals that there is _____

Answer 22

Demonstration of the engulfment of bacteria

Question 23

Negative result reveals that there is _____

Answer 23

No engulfment of bacteria

Question 24

The process by which specialized cells engulf and destroy foreign particles such as microorganisms or damaged cells.

Answer 24

Phagocytosis

Question 25

These are the most important phagocytic cells.

Answer 25

Macrophages
Neutrophils

Question 26

Complete the Statement:

A mixture of _____ and _____ is incubated and examined for the presence of engulfed bacteria.

Answer 26

Bacteria
Phagocytes

Question 27

Complete the Statement:

A mixture of bacteria and phagocytes is _____ and _____ for the presence
of engulfed bacteria.

Answer 27

Incubated
Examined

Question 28

Complete the Statement:

A mixture of bacteria and phagocytes is incubated and examined for the presence
of _____.

Answer 28

Engulfed bacteria

Question 29

Complete the Statement:

A mixture of bacteria and phagocytes is incubated and examined for the presence
of engulfed bacteria. This simple procedure may be useful in supporting the diagnosis of _____ in conjunction with clinical signs and symptoms.

Answer 29

Impaired Neutrophilic Function

Question 30

Sources of Error:

Answer 30

• Blood specimen is not fresh
• If a coagulase-positive Staphylococcus specimen is used

Question 31

The procedure may produce _____ if the blood specimen is not fresh or if a coagulase-positive Staphylococcus specimen is used.

Answer 31

False-negative Result

Question 32

The bacteria must be _____ and not _____ for the test to be positive.

Answer 32

Intracellular
Extracellular

Question 33

Limitations of the Procedure:

Answer 33

This is the simple screening procedure for engulfment. The presence of engulf bacteria does not demonstrate that the bacteria have been destroyed.