Sectioning

Question 1

Microtome model that we use in the lab

Answer 1

Leica RM2125 RTS Microtome

Question 2

Measurement of sections produce by the Leica RM2125 RTS Microtome

Answer 2

0 - 60um

Question 3

2 dot meaning

Answer 3

Initial Sectioning

Question 4

Initial sectioning measurement

Answer 4

25um

Question 5

1 dot meaning

Answer 5

Final Sectioning

Question 6

Final sectioning measurement

Answer 6

10um

Question 7

Floatation bath temp

Answer 7

5-10C below melting point of paraffin wax

Question 8

Most commonly used adhesive

Answer 8

Meyer’s Egg Albumin

Question 9

Ratio concentration for Meyer’s Egg Albumin

Answer 9

1:1

Question 10

Meyer’s Egg Albumin composition

Answer 10

1mL Egg white & 1mL Glycerin

Question 11

Added to Meyer’s Egg Albumin to prevent molds

Answer 11

Phenol

Question 12

Adding Phenol to Meyer’s Egg Albumin will allow the slide to last for how long?

Answer 12

3 - 4 months

Question 13

Other names for Sectioning

Answer 13

Microtomy
Cutting

Question 14

The process where tissues are cut into uniformly thin slices to facilitate microscopic studies

Answer 14

Sectioning

Question 15

Most paraffin embedded tissues are sectioned to what measurement

Answer 15

4 - 8um

Question 16

Histotechnologists are the _______ of the laboratory

Answer 16

artists

Question 17

Common artifacts introduced in sectioning

Answer 17

Tearing
Ripping
Holes
Folding

Question 18

4 types of microtome knives

Answer 18

Standard Thick Metal
Thin Disposable Blades
Glass Knives
Diamond Knives

Question 19

This knife allows for sharpening to your liking and is considered to be expensive

Answer 19

Standard thick metal

Question 20

Does the same job as the standard thick metal knife, and probably better, for a much lesser cost

Answer 20

Thin disposable blades

Question 21

Glass knives are for tissue sections embedded in what types of plastic materials

Answer 21

Methacrylate
Araldite
Epon

Question 22

A glass knife can section down to what measurement

Answer 22

About 1um

Question 23

Knife used for electron microscopy

Answer 23

Diamond knives

Question 24

Diamond knife can section to what measurement

Answer 24

About 0.25um

Question 25

Angle that prevents the contact between the knife facet and the face of the block

Answer 25

Clearance angle

Question 26

Recommended clearance angle

Answer 26

0 - 15 degrees

Question 27

Bevel angle is aka?

Answer 27

Facet angle

Question 28

The angle between the two facets that form the cutting edge

Answer 28

Bevel Angle

Question 29

Facet angle

Answer 29

approx. 35 degrees

Question 30

Consequences of incorrect knife angle will vary on the differences in what factors?

Answer 30

Specimen types Fixation Processing Qualities Knife Flexibility

Question 31

Causes if the ribbon is crooked rather than straight

Answer 31

- edges of the block face are not parallel to each other - uneven knife edge

Question 32

Causes if the ribbon will not form and each section comes off separately

Answer 32

- Dull blade edge - Knife has wrong tilt - Edges of paraffin block face are crumbled or rounded

Question 33

Causes if sections are compressed or folded

Answer 33

- dull knife - knife angle is set too close to the vertical - knife is clogged with paraffin - sections are too thin - paraffin is too soft due to warm room - paraffin is contaminated with xylene

Question 34

Causes if the specimen crumbles or falls out of the paraffin section

Answer 34

- tissue is inadequately dehydrated or cleared - tissue is too hard and compact - tissue was in the paraffin oven too long and at too high temp - tissue is too hard for paraffin matrix

Question 35

Causes if the ribbon continuously splits or scratches vertically

Answer 35

- crystals or dirt particles are caught on the knife edge or on paraffin face - nicked knife edge

Question 36

Causes if the ribbon wraps itself around your finger, the knife blade, or anything else

Answer 36

- static electricity

Question 37

In prepping the tissue block for sectioning, trimming the lateral surface should result in resembling what shape

Answer 37

Trapezoid

Question 38

After resulting in a trapezoid resembling shape, the tissue block must still be surrounded by a frame of how much wax

Answer 38

2-3mm

Question 39

Wipe the microtome blade using what solution?

Answer 39

Chloroform or Xylene

Question 40

In the blade preparation, the angle of the center line of the blade with the face of the block should be about how many degrees

Answer 40

20 degrees

Question 41

During sectioning, students should start at how many microns

Answer 41

10um

Question 42

In sectioning, how much paraffin should be allowed to move up and down until the ribbon begins to bow up

Answer 42

first inch or two

Question 43

How long must the ribbon be before detaching it form the edge of blade

Answer 43

8 inches

Question 44

How must we detach the ribbon from the edge of the blade

Answer 44

In an upward stroke

Question 45

How should we brush the blade

Answer 45

away from the cutting of the blade

Question 46

What should the orientation of the ribbon be when laying it in the ribbon box

Answer 46

Dull surface up

Question 47

When mounting sections, slide should be rinsed in what

Answer 47

95% ethanol

Question 48

What should be used to write information on the slide

Answer 48

Diamond-tipped pencil Lead pencil

Question 49

Paraffin expands to how many percent

Answer 49

20%

Question 50

What is the measurement of the cover slip used after staining

Answer 50

22mm x 22mm

Question 51

At what temperature should the slide warmer be

Answer 51

40 - 45C