SEDIMENT STAINS Flashcards
A supravital stain consisting of Crystal violet and safranin
Sternheimer-Malbin
Sternheimer-Malbin
Delineates structure and contrasting colors of the nucleus and cytoplasm
Sternheimer-Malbin
Identifies WBCs, epithelial cells, and casts
Sternheimer-Malbin
A metachromatic supravital stain
0.5%Toluidine blue
Enhances nuclear detail
0.5%Toluidine blue
Differentiates WBCs and renal tubular epithelial (RTE) cells
0.5%Toluidine blue
Distinguishes RBCs from WBCs, yeast, oil droplets, and crystals
2% acetic acid
Lyses RBCs and enhances nuclei of WBCs
2% acetic acid
Stain triglycerides and neutral fats orange-red
Lipid Stains: Oil Red O and Sudan III
Identifies free fat droplets and lipid containing cells and casts
Lipid Stains: Oil Red O and Sudan III
Differentiates gram-positive and gram negative bacteria
Gram stain
Identifies bacterial casts
Gram stain
Methylene blue + EosinY
Hansel stain
Stains eosinophilic granules; Identifies urinary eosinophils
Hansel stain
Stains structures containing iron
Identifies urinary eosinophils
Identifies yellow-brown granules of hemosiderin in cells and casts
Identifies urinary eosinophils
Modified Sternheimer Malbin
The dye is absorbed well by WBCs,
epithelial cells, and casts, providing
clearer delineation of structure and
contrasting colors of the nucleus and
cytoplasm
Sedi and KOVA stain
Hyaline cast appears as pink
Motile bacteria are unstained
Non-motile bacteria stains purple
T.vaginalis stains Light blue-green
Sedi and KOVA stain
