Semen Flashcards

1
Q

Maturation of spermatozoa takes place in the:

A. Sertoli cells
B. Seminiferous tubules
C. Epididymis
D. Seminal vesicles

A

Epididymis

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2
Q

Enzymes for the coagulation and liquefaction of semen are produced by the:

A. Seminal vesicles
B. Bulbourethral glands
C. Ductus deferens
D. Prostate gland

A

Prostate gland

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3
Q

The major component of seminal fluid is:

A. Glucose
B. Fructose
C. Acid phosphatase
D. Citric acid

A

Fructose

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4
Q

If the first portion of a semen specimen is not collected, the semen analysis will have which of the following?

A. Decreased pH
B. Increased viscosity
C. Decreased sperm count
D. Decreased sperm motility

A

Decrease sperm amount

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5
Q

Failure of laboratory personnel to document the time a semen sample is collected primarily affects the interpretation of semen:

A. Appearance
B. Volume
C. pH
D. Viscosity

A

Viscosity

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6
Q

Liquefaction of a semen specimen should take place within:

A. 1 hour
B. 2 hours
C. 3 hours
D. 4 hours

A

1 hour

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7
Q

A semen specimen delivered to the laboratory in a condom has a normal sperm count and markedly decreased sperm motility. This indicates:

A. Decreased fructose
B. Antispermicide in the condom
C. Increased semen viscosity
D. Increased semen alkalinity

A

Antispermicide in the condom

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8
Q

An increased semen pH may be caused by:

A

Prostatic infection
Decreased prostatic secretions
Decreased bulbourethral gland secretions

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9
Q

Proteolytic enzymes may be added to semen specimens to:

A. Increase the viscosity
B. Dilute the specimen
C. Decrease the viscosity
D. Neutralize the specimen

A

Decrease the viscosity

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10
Q

The normal sperm concentration is:

A. Less than 20 million/uL
B. More than 20 million/mL
C. Less than 20 million/mL
D. More than 20 million/uL

A

More than 20 million/mL

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11
Q

Given the following information, calculate the sperm con-centration: dilution, 1:20; sperm counted in five RBC squares on each side of the hemocytometer, 80 and 86; volume, 3 mL.

A. 80 million/mL
B. 83 million/mL
C. 86 million/mL
D. 169 million/uL

A

83 million/mL

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12
Q

Using the above information, calculate the sperm concentration when 80 sperm are counted in 1 WBC square and 86 sperm are counted in another WBC square.

A. 83 million/mL
B. 166 million per ejaculate
C. 16.6 million/mL
D. 50 million per ejaculate

A

16.6 million/mL

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13
Q

The primary reason to dilute a semen specimen before performing a sperm concentration is to:

A. Immobilize the sperm
B. Facilitate the chamber count
C. Decrease the viscosity
D. Stain the sperm

A

Immobilize the sperm

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14
Q

When performing a sperm concentration, 60 sperm are counted in the RBC squares on one side of the hemocy-tometer and 90 sperm are counted in the RBC squares on the other side. The specimen is diluted 1:20. The:

A. Specimen should be rediluted and counted
B. Sperm count is 75 million/mL
C. Sperm count is greater than 5 million/mL
D. Sperm concentration is abnormal

A

Specimen should be rediluted and counted

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15
Q

Sperm motility evaluations are performed:

A. Immediately after the specimen is collected
B. Within 1 hour of collection
C. After 3 hours of incubation
D. At 6-hour intervals for 1 day

A

Within 1 hour of collection

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16
Q

The percentage of sperm showing average motility that is considered normal is:

A. 25%
B. 50%
C. 60%
D. 75%

A

50%

17
Q

The purpose of the acrosomal cap is to:

A. Penetrate the ovum
B. Protect the the nucleus
C. Create energy for tail movement
D. Protect the neckpiece

A

Penetrate the ovum

18
Q

The sperm part containing a mitochondrial sheath is the:

A. Head
B. Neckpiece
C. Midpiece
D. Tail

A

Midpiece

19
Q

All of the following are associated with sperm motility except the:

A. Head
B. Neckpiece
C. Midpiece
D. Tail

A

Head

20
Q

The morphologic shape of a normal sperm head is:

A. Round
B. Tapered
C. Oval
D. Amorphous

A

Oval

21
Q

Normal sperm morphology when using the WHO criteria is:

A. >30% normal forms
B. <30% normal forms
C. >15% abnormal forms
D. <15% normal forms

A

> 30% normal forms

22
Q

Additional parameters measured by Kruger’s strict morphology include all of the following except:

A. Vitality
B. Presence of vacuoles
C. Acrosome size
D. Tail length

A

Vitality

23
Q

Round cells that are of concern and may be included in sperm counts and morphology analysis are:

A

Leukocytes and spermatids

24
Q

If 5 round cells per 100 sperm are counted in a sperm morphology smear and the sperm concentration is 30 million, the concentration of round cells is:

A. 150,000
B. 1.5 million
C. 300,000
D. 15 million

A

1.5 million

25
Q

Following an abnormal sperm motility test with a normal sperm count, what additional test might be ordered?

A. Fructose level
B. Zinc level
C. MAR test
D. Eosin-nigrosin stain

A

Eosin-nigrosin stain

26
Q

Follow-up testing for a low sperm concentration would include testing for:

A. Antisperm antibodies
B. Seminal fluid fructose
C. Sperm vitality
D. Prostatic acid phosphatase

A

Seminal fluid fructose

27
Q

The immunobead test for antisperm antibodies:

A. Detects the presence of male antibodies
B. Determines the presence of IgG, IgM, and IgA antibodies
Determines the location of antisperm antibodies
D. All of the above

A

Detects the presence of male antibodies
Determines the presence of IgG, IgM, and IgA antibodies
Determines the location of antisperm antibodies

28
Q

Measurement of a-glucosidase is performed to detect a disorder of the:

A. Seminiferous tubules
B. Epididymis
C. Prostate gland
D. Bulbourethral glands

A

Epididymis

29
Q

A specimen delivered to the laboratory with a request for prostatic acid phosphatase and glycoprotein p30 was collected to determine:

A. Prostatic infection
B. Presence of antisperm antibodies
C. A possible rape
D. Successful vasectomy

A

A possible rape

30
Q

Following a negative postvasectomy wet preparation, the specimen should be:

A. Centrifuged and reexamined
B. Stained and reexamined
C. Reported as no sperm seen
D. Both A and B

A

Centrifuged and reexamined

31
Q

Standardization of procedures and reference values for semen analysis is primarily provided by the:

A. Manufacturers of instrumentation
B. WHO
C. Manufacturers of control samples
D. Clinical Laboratory Improvement Amendments

A

WHO