Semen Collection Techniques

Question 1

Performed by EE or through the use of AV

Answer 1

Bovine

Question 2

Digital massage of _______ through the rectum produces ________, semen of variable quality and is infrequently employed

Answer 2

Bovine:
Digital massage of accessory glands through the rectum produces erratic responses, semen of variable quality and is infrequently employed

Question 3

Bovine:
Semen collected from EE are usually___________________

Answer 3

greater in volume,
higher pH,
lower concentration of
spermatozoa and fructose per mL of semen

Question 4

Bovine:
In EE, probes with three ventrally located electrodes are superior to the four-electrode model due to ________________

Answer 4

a more focused stimulation of the reproductive tract, less
muscular rigidity, and a firmer foot placement

Question 5

Bulls are restrained in a chute, then the probe is
lubricated, inserted into the evacuated rectum and an
assistant holds the yoke to prevent the bull from
expelling the probe

Answer 5

EE in bulls

Question 6

EE in bulls:
Semen is maintained at_____

Answer 6

Semen is maintained at 30-35 deg. C

Question 7

device should be of proper length, and semen should be ejaculated near the directing cone rather than in the liner to avoid sperm death by high temperature

Answer 7

AV in Bovine

Question 8

AV in Bovine:
If additional ejaculates are desired, the bull must be
rested for _______before repeating with a new
AV

Answer 8

10 to 15 mins

Question 9

AV is preferred, as EE is not tolerated

Answer 9

Equines (Stallions)

Question 10

Rigid AV uses rubber or disposable liners and collecting cones, whereas cones are an integral part of the soft AV

Answer 10

Equine

Question 11

Equine:
Two types of AV are used

Answer 11

one with a more rigid outer
casing, and one with two layers of soft rubber with a small harness for carrying support

Question 12

Equine:
Recommended water temperature is ________

Answer 12

between 45-50 deg. Celsius

Question 13

Sterile plastic bottles can be used as ________, filters can be added to remove the gel fraction from the ejaculate

Answer 13

Equine:
collection vials

Question 14

A __________ which the stallion is accustomed to is used in semen collection

Answer 14

mounting mare / mounting phantom

Question 15

The mare is _________ for sanitation and prevent penile abrasions during collection

Answer 15

prepared by clipping or wrapping the tail

Question 16

Equine:
______ is thoroughly washed and dried before
mounting

Answer 16

Perineal area

Question 17

Stallion is teased to _______, then the penis is washed and the ________removed

Answer 17

to produce erection,
smegma removed

Question 18

Equine:
___________occurs after
ejaculation and continues until dismount

Answer 18

Flagging (up and down motion of stallion’s tail)

Question 18

As the stallion moves toward the mare, the penis is guided towards the AV by

Answer 18

grasping the penile shaft

Question 19

Equine:
Mare’s head is turned partially toward the stallion as he dismounts to prevent __________

Answer 19

kicking and injury

Question 20

Semen is collected via EE (common) or AV

Answer 20

Ovine

Question 21

Ovine:
In EE, a __________ is inserted into the rectum of the ram and semen collection is similar to the bull

Answer 21

three-electrode rectal probe

Question 22

Ovine:
In EE, two approaches may be

Answer 22

1) ram remains standing
supported by a crate and removable boards to restrict
movement, or

2) lateral recumbency, which facilitates easier exteriorization of penis but requires physical restraint

Question 23

In AV, an immobile, non-estrus ewe is used, restrained in a
head catch and side restraining bars

Question 24

Ovine: Ideal temperature for semen collection is _________

Answer 24

between 40-45 deg. Celsius

Question 25

In AV, _________is used, restrained in a head catch and side restraining bars

Answer 25

an immobile, non-estrus ewe

Question 26

Ideal temperature for semen collection is between _______

Answer 26

between 40-45 deg. Celsius

Question 27

EE is more preferable in the buck for semen evaluation, AV is preferable for semen for AI

Answer 27

Caprine semen collection techniques

Question 28

EE is done via standing or lateral recumbency similar to rams (three-electrode, 3-4 secs followed by a 2-3 secs rest phase, at 0-60 mV)

Answer 28

Caprine

Question 29

Caprine: In AV, an untrained buck requires ________ though ________may only require a non-estrus doe

Answer 29

In AV, an untrained buck requires a doe in estrus, though trained ones may only require a non-estrus doe

Question 30

Uses either EE, direct manipulation (DM, more preferred) via fingers of a gloved hand, or with an AV

Answer 30

Porcine

Question 31

Porcine: AV requires ___________

Answer 31

teaser sow, estrous sow or phantom

Question 31

Porcine: _______ are recommended since latex gloves are spermicidal

Answer 31

Vinyl gloves

Question 32

Porcine: AV for collection is similar with other species, with the interior of the casing between ________and should collect approx.__________ of semen

Answer 32

45-50 deg. Celsius and should collect approx. 400-500 mL of semen

Question 33

Porcine: Collection by EE requires ___________

Answer 33

general anesthesia (thiopental)

Question 34

Porcine: EE requires an evacuated rectum, with the probe having_______

Answer 34

circular rather than longitudinal electrodes

Question 35

Porcine: Power is administered for______, followed by a rest phase of _______

Answer 35

Power is administered for 5-7 secs., followed by a rest phase of 5-10 secs

Question 36

Porcine: The first collection is sperm-poor, and is discarded, followed by the sperm rich fraction intermixed with the gel portion

Answer 36

True