Seminar Flashcards
(33 cards)
Also known as Coulter principle
Electronic impedance
Electronic impedance is based on the detection and measurement of changes in electrical resistance produced by cells as they traverse a large aperture
T or F
F
Small aperture
Electronic impedence is electrical resistance between the two electrodes, or impedance in the current, occurs as the cells pass through the sensing aperture, causing voltage pulses that are measurable
T or F
T
In electronic impedance:
The number of pulses is _____________ to the numbers of cells counted
proportional
In electronic impedance:
The height of the voltage pulse is ___________ proportional to cell volume.
directly
In electronic impedance:
Blood sample is distributed into two channels: ____/PLT and WBC/____
RBC
HGB
In electronic impedance:
RBC / Platelet Channel:
The machine determines the component if cell volumes are:
− 36-360 fL = ____
− 2-20 fL = _____
RBCs
Platelets
In electronic impedance:
WBC / Hemlglobin Channe
Adds lysing agent to remove RBCs
T or F
T
In electronic impedance:
WBC / Hemlglobin Channe
3-part WBC differential count are:
− 35-90 fL = ______________
− 91-160 fL = _____________
− 161-450 fL = ______________
Lymphocytes
Monocytes
Granulocyte
When the RBCs are lysed, the hemoglobin comes out.
− The standard method for Hgb measurement is the ______________________________ measured
spectrophotometrically at 540 nm
Cyanmethemoglobin method (HiCN),
In RBC histogram:
The x-axis represents the cell volume.
The y-axis represents the relative number or frequency
T or F
T
NOTE:
RBC histogram is basically MCV that is being plotted in a graph
Usually used in conjunction with electronic impedance
Radiofrequency
RF + EI is now capable of _-part differential count
5
The 5 part differential count includes?
neutrophil, eosinophil, basophil,
monocyte, and lymphocyte
3-part differential count includes?
lymphocyte, monocyte, and granulocytes
The cell interior density is proportional to pulse height or change in the RF signal
T or F
T
Conductivity is determined using a low frequency electromagnetic probe that provides information on the cell internal constituents.
T or F
F
High
a principle that is the detection of scattered rays and their conversion into electrical signals is accomplished by photodetectors at specific angles
OPTICAL LIGHT SCATTER
______________ light scatter (__degrees) correlates with cell volume.
____ light scatter (___degrees) correlates with degree of internal complexity
Forward angle, 0
Side, 90
Other name of optical light scatter?
Laser based technology
Laser based technology is capable of 3 part differential count
T or F
F
5
Error in automation:
Cold agglutinins may affect what parameter due to clumping at low temp?
_______ RBC
_______ MCHC
_______ MCV
Decrease
Increase
Increase
Error in automation:
Lipemia, icteric sample may affect what parameter ?
_______ Hgb
_______ MCHC
All increase