Serial dilutions

Question 1

Serial dilutions are created by taking a series of

Answer 1

dilutions of a stock solution. The concentration decreases by the same quantity between each test tube

They can either be ‘doubling dilutions’ (where the concentration is halved between each test tube) or a desired range (e.g. 0, 2, 4, 6, 8, 10 mmol dm-3)

Question 2

Serial dilutions are completed to create a standard to compare unknown concentrations against
The comparison can be

Answer 2

Visual
Measured through a calibration/standard curve
Measured using a colourimeter

Question 3

They can be used when

Answer 3

Counting bacteria or yeast populations
Determining unknown glucose, starch, protein concentrations

Question 4

quantities of glucose stock solution determined by what equation

Answer 4

desired concentration / concentration of stock x volume wanted