Sexual Assault Evidence

Question 1

Seminal Plasma

Answer 1

Composed of 
Proteins
Salts
Sugars
Lipids
Enzymes (Acid phosphatase)
Nutrients 
Hormones
Basic amines (spermine)
P30 (prostate specific antigen-PSA)
Choline 
Cellular material
Flavins (source of flourescence under UV -seen using ALS)

Question 2

Fluid delivered through penis in ejaculation

Answer 2

Semen

*spermatozoa (cellular component) = plural

Question 3

Acid phosphatase (AP) and Prostate Specific Antigen (PSA) both come from ______

Answer 3

Prostate gland

Question 4

Seminiferous tubles

Answer 4

Long thread like tubes found in each of the testicles where spermatozoa are produced

Question 5

Coagulation

Answer 5

Putting together making gel like substance

Question 6

Seminal plasma secretions

Answer 6

Testes
Epididymides
Seminal vesicles 
Prostate
Cowper's glands

Question 7

Spermatozoon

Answer 7

Singular

Question 8

Half sperm = X

Half sperm = Y

Answer 8

Haploid cell

Question 9

Morphology of sperm

Answer 9

Head -contains nucleus & acrosome

Tail and Midpiece- contain mechanisms and enzymatic materials for motility

Question 10

• Contains mitochondria
• Each sperm mitochondrion carries multiple copies of paternal mitochondria genome
• energy for sperm flagellar motility
• joins head and tail

Answer 10

Midpiece (neck)

Question 11

Mobility or motility of spermatozoa

Answer 11

Tail

Question 12

NFSTC

Answer 12

National forensic science technology center

Question 13

Protamines

Answer 13

Arginine rich nuclear protein that replaces histones late in haploid phase of soermatogenesis

Question 14

Two types of protamines are______

Answer 14

P1 & P2

Question 15

Aspermia

Answer 15

No ejaculation (absence of spermatozoa)

• infertility
• drug use
• prostate surgery

Question 16

Azoospermia

Answer 16

Lack of sperm cells in the semen (no spermatozoa)

• temporary or permanent
• vasectomy
• drugs
• alcoholism
• high fever

Question 17

Oligospermia (asthenozoospermia)

Answer 17

Fewer number of spermatozoa

Question 18

Teratozoospermia

Answer 18

Increased abnormal forms of sperm

Question 19

Oligoasthenoteratozoospermia

Answer 19

Spermatozoa variables all subnormal (OAT)

Question 20

Leucocytospermia

Answer 20

Increased leukocytes in semen

Question 21

Necrozoospermia

Answer 21

All sperm non viable or non motile

Question 22

Globozoospermia

Answer 22

Sperm heads lack acrosome caps and can’t fertilize

rare*

Question 23

Phagocytic

Answer 23

Eats other cells

Question 24

Protease

Answer 24

Break up of proteins

Question 25

high concentration in semen and liquefies seminal fluid * serine protease * produced by epithelial cells & found in seminal fluid

Answer 25

PSA

Question 26

Protease

Answer 26

Break up of proteins

Question 27

high concentration in semen and liquefies seminal fluid * serine protease * produced by epithelial cells & found in seminal fluid

Answer 27

PSA

Question 28

Protease

Answer 28

Break up of proteins

Question 29

high concentration in semen and liquefies seminal fluid * serine protease * produced by epithelial cells & found in seminal fluid

Answer 29

PSA

Question 30

Y shaped proteins

Answer 30

Immunoglobulin G (IgG)

Question 31

ELISA

Answer 31

Enzyme linked immunosorbent assay

Question 32

RSID

Answer 32

Rapid Stain Identification Semen Test

Question 33

ABAcard P30 detects_____

Answer 33

PSA

Question 34

RSID detects ______

Answer 34

Semenogelin (Sg)

Question 35

3 major gel-forming proteins

Answer 35

Fibronectin Semenogelin I Semenogelin II

Question 36

Semenogelin I

Answer 36

Disulfide linked complex of ~52-kDa protein

Question 37

Semenogelin II

Answer 37

Two forms of an Sg I related protein of ~71 and ~76 kDa

Question 38

Seminiferous tubules

Answer 38

Where sperm is being produced

Question 39

Epididymis

Answer 39

A coiled tube next to each testis and receives sperm from the seminiferous tubules

Question 40

Three phases of spermatogenesis

Answer 40

Spermatocytogenesis Diploid cell meiosis Spermiogenesis

Question 41

Spermatocytogensis

Answer 41

Immature spermatogonia undergo mitotic division *where mitosis takes place

Question 42

Diploid cells -Meiosis

Answer 42

Diploid number present in first phase becomes haploid, 23 chromosomes *secondary spermatocytes (haploid spermatids)

Question 43

Spermiogenesis

Answer 43

Spermatids undergo morphological changes *final phase-spermatids are haploid, see acrosome form & elongated

Question 44

Spermiogenesis

Answer 44

Midpiece - mitochondria Acrosome - enzymatic knife used to penetrate the oocyte Nucleus - haploid chromosomes

Question 45

Vas deferens

Answer 45

* Main vesicle for carrying sperm * travels out scrotum--> into abdomen--> & passes behind urinary bladder--> expands to form expanded end part (ampulla) Ampulla =bulb shape

Question 46

Urethra

Answer 46

Carries semen (sperm) through penis during intercourse and during urination passes through it *two different functions, do not occur at the same time

Question 47

SRY

Answer 47

Sex determining region *Y is smaller then X

Question 48

ZFX

Answer 48

Zinc fingerprint protein * located on X chromosome * extremely important in humans

Question 49

Danes method

Answer 49

Keratin Prekeratin Mucin

Question 50

Ayoub-Shklar

Answer 50

Differentiate skin epithelial cells from buccal and vaginal

Question 51

VAP

Answer 51

Vaginal acid phosphatase

Question 52

SAP

Answer 52

Seminal acid phosphatase

Question 53

ELISA

Answer 53

Enzyme linked immunosorbent assay

Question 54

KPIC

Answer 54

Kernechtrot Picroindigocarmine stain

Question 55

3 methods Fertility and Sterility

Answer 55

Shorr Papanicolau (papsmear) Testsimplets