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Microbio Lab Test 1 > Simple Stain > Flashcards

Flashcards in Simple Stain Deck (7)
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1
Q

Simple Stain

A

Uses only one stain

2
Q

Most Common Stains for Simple Stain

A

Methylene blue and crystal violet

3
Q

Two types of simple stains

A

Basic (Positive) and Acidic (Negative)

4
Q

Basic Stain

A
  • Positive Stain
  • Colors actual bacteria
  • Bacteria are negatively charged
  • Stain contains chromophores
  • Basic stain contains cations
  • Stain is attracted to bacteria
  • Requires heat fixation: possible distortion of cells
5
Q

Charge of bacteria

A

Slightly negative

6
Q

Chromophores

A

Color-bearing ions

7
Q

Acidic Stain

A
  • Negative Stain
  • Colors background
  • Bacteria are negatively charged
  • Stain contains chromophores
  • Acidic stain contains anions
  • Stain is repelled from bacteria
  • Does not require heat fixation: no distortion of cell shape or size
  • Stain used: Nigrosin stain