Simplified Flashcards
(8 cards)
Title
The effect of enzyme concentration on substrate reaction.
Aim
To investigate the effect of increasing enzyme concentration on Catalase reaction with Hydrogen peroxide.
Underlying Biology
*The presence or abundance of certain enzymes end-product can control metabolic pathways. Feedback…
*The active site of an enzyme has a strong affinity for the complementary substrate. This means..
*Enzyme activity is controlled my molecules known as inhibitors. these inhibitors come in two forms and use different methods..
*Non-Competitive inhibitors vairy slightly in their method of inhibition. Unlike Competitive inhibitors, they do not..
Experimental Procedure
*In each of the 3 test tubes there were different concentrations of Catalase (Enzyme). There was then set volumes of..
*Catalase solution concentration
*Fairy Liquid
*Hydrogen Peroxide
*Stop watch
*Ruller
*Repeated 3 times for averages
Analysis
Compare my results to the reference (e.g its clear that enzyme concentration has a … from my own data i calculated (difference/original x 100)) online source showed similar results of
*From the results I have gathered from both my own experiment as well as my experimental research reference, it is clear that catalase (enzyme) concentration has a direct effect on the volume of foam …
*Percentage Inc = difference/original x 100:
*This shows the reaction rate increase substantially by 1631 % as the…
*The results from the online source showed similar results of increasing catalase…
Conclusion
*As the enzyme (Catalase) concentration is increased, the substrate reaction also increases.
*This is shown by the volume of foam production increasing, As the catalase..
Evaluation
*In evaluation of my experiment and the results I measured I believe that experiment and data gathered was pretty reliable. This is because I adopted some recognised experimental producers to ensure the integrity of my data.
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