Slide Prep

Question 1

The study of cells and their surrounding envi (extracellular components) and their organization into tissues and organs

Answer 1

Histology

Question 2

The study of indiv cells

Answer 2

Cytology

Question 3

Type of electron microscope that emits beams that pass over the tissue surface

Answer 3

Scanning EM

Question 4

Type of electron microscope that emits beams that penetrate the tissue

Shows more detail

Answer 4

Transmission EM

Question 5

Eyepiece magnification

Answer 5

10x

Question 6

Scanner magnification

Answer 6

4x

Question 7

LPO magnification

Answer 7

10x

Question 8

HPO magnification

Answer 8

40x

Question 9

OIO magnification

Answer 9

97-100x

Only used for peripheral blood and bone marrow

Question 10

Angstrom value

Answer 10

0.1 nm / 10^-10 m

Question 11

Diameter of HPO field of view

Answer 11

0.4 mm

Question 12

Diameter of RBC

Answer 12

7.74 um

Question 13

Steps of slide preparation

Answer 13

1. Fixation
2. Embedding
   a. Dehydration
   b. Clearing
3. Staining

Question 14

Step that preserves tissue morphology and architecture and prevents cell digestion

Answer 14

Fixation

Question 15

2 examples of fixatives

Answer 15

Formaldehyde

Glutaraldehyde

Question 16

Step for obtaining a rigid consistency for sectioning

Answer 16

Embedding

Question 17

Two steps that precede embedding

Answer 17

Dehydration

Clearing

Question 18

Reagent used for dehydration

Answer 18

EtOH (40, 60, 80, 90%)

Question 19

Lipid solvents that can be used for bathing in the clearing step

Answer 19

Benzene or xylene

Water and alcohol not miscible with paraffin

Xylene miscible with paraffin

Question 20

Machine used for sectioning

Answer 20

Microtome

Question 21

T/F

Slides for alight microscope can be used for an electron microscope

Answer 21

False

Question 22

Basic dyes react with?

Acidic dyes react with?

Answer 22

Acidic structures (basophilic)

Basic structures (acidophilic)

Question 23

Structures stained by hematoxylin

And also color

Answer 23

Blue

Nucleus (DNA, RNA)
Acidic regions of cytoplasm
Cartilage matrix
Ribosomes

(Acidic structures)

Question 24

Structures stained by eosin

And color

Answer 24

Pink

Basic regions of cytoplasm
Collagen fibers

Question 25

Structures stained by PAS And color

Answer 25

Magenta Glycogen Carb rich molecules

Question 26

Structures stained by Wright and Giemsa stain And color

Answer 26

Pink - erythrocytes - eosinophil granules Purple - leukocyte nuclei - basophil granules Blue - cytoplasm of monocytes and lymphocytes

Question 27

Structures stained by Orcein Elastic Stain And color

Answer 27

Brown Elastic fibers

Question 28

Structures stained by silver stain And color

Answer 28

Black Reticular fibers

Question 29

Structures stained by iron hematoxylin And color

Answer 29

Black Muscle striations Nuclei Erythrocytes

Question 30

Structures stained by Massom’s Trichome And color

Answer 30

Dark blue - nuclei Red - muscle - cytoplasm Light blue - collagen