Small Pleomorphic Gram (-) Fastidious Coccobacilli

Question 1

haemophilus: catalase (+) spps

Answer 1

h. influenzae, h. hemolyticus

Question 2

haemophilus: preferred incubation

Answer 2

35-37C at 5-10% CO2

Question 3

very susceptible to drying and extreme temperatures

Answer 3

haemophilus

Question 4

haemophilus: normally inhabit the URT & oral cavity except ___

Answer 4

H. ducreyi

Question 5

demonstrates satellite phenomenon

Answer 5

haemophilus

Question 6

charactersitics of x factor

Answer 6

hemin, heat stable, aka protoporphyrin 9

Question 7

characteristics of v factor

Answer 7

isolated from horse blood, heat labile, AKA coenzyme 1 / nicotinamide adenine dinucleotide

Question 8

v factor can be supplied by

Answer 8

potatoes, yeast, staph, pneumo, gono (cocci)

Question 9

Pasteurella: indole (+) except

Answer 9

P. bettyae

Question 10

Pasteurella: Appearance

Answer 10

safety pin

Question 11

Pasteurella: susceptible to ___

Answer 11

penicillin

Question 12

VF of Pasteurella

Answer 12

endotoxin & capsule

Question 13

Bordetella: NM except _____

Answer 13

B. Bronchieseptica

Question 14

Bordetella: oxidase (+) except ____

Answer 14

B. parapertussis

Question 15

Bordetella: urease (+) except

Answer 15

B. pertussis

Question 16

exhibits mercury colonies

Answer 16

Bordetella

Question 17

Bordetella: Gram stain

Answer 17

safranin or basic fuchsin (.2%) as counterstain for 2 mins

Question 18

replicates in ciliated respiratory endothelial cells

Answer 18

Bordetella

Question 19

Bordetella: Growth factors

Answer 19

methionine, cysteine, nicotinic acid

Question 20

VF of Bordetella

Answer 20

pertussis toxin, adenylyl cyclase, tracheal cytotoxin and dermonecrotic toxin, pertactin and fimbriae

Question 21

Brucella: result on bap

Answer 21

gamma hemolytic

Question 22

Brucella: H2s (+) except

Answer 22

B.canis

Question 23

normal flora of urinary tract & GIT of shep

Answer 23

Brucella

Question 24

listed as category b agent for bioterrorism

Answer 24

Brucella

Question 25

sand appearance

Answer 25

Brucella

Question 26

placental tissued used as a growth factor

Answer 26

erythritol

Question 27

dye inhibition used in brucella and results:

Answer 27

thionine & fuchsin dye inhibition [(+) = not inhibited // (-) = inhibited]