SMEAR PREP

Question 1

These are smears prepared directly from the clinical specimen

Answer 1

Direct smears

Question 2

T or F: Findings in the direct smear may confirm or reject a diagnosis.

Answer 2

True

Question 3

T or F: direct smear allows diagnosis of the predominating type of organism

Answer 3

False: diagnosis - visualization

Question 4

T or F: An organism that does not appear in the culture is seen in the direct smear. This is a lack of specificity of the gram stain.

Answer 4

True

Question 5

What are the possible explanations for an organism to appear in direct smear but not in culture?

Answer 5

1. Organism grows slowly, will appear if the culture is held longer
2. patient received sufficient treatment (antibiotics) to prevent growth of the organism (nonviable)
3. Smear performed incorrectly, over or under-decolorized.

Question 6

T or F: It is possible that no organisms are seen in the direct smear but organisms still grow

Answer 6

True - due to lack of sensitivity of the gram stain compared with culture

Question 7

T or F: don’t roll the swab across the slide to avoid destruction of cellular entities

Answer 7

False - Roll it across

Question 8

Prepare smears of concentrated centrifuge specimens by using a sterile, ______?

Answer 8

plastic transfer pipette

Question 9

This is done by spreading a thin film of Bacteria on the surface of a clean glass slide

Answer 9

Smears from CULTURES (Subculture smears)

Question 10

T or F: In smears from liquid media, place a loopful of culture in the square. Unless growth is exceptionally heavy, spread.

Answer 10

False - If heavy, do not spread

Question 11

In smearing blood culture bottles, use __ slide per specimen. Then, decontamination the top of the bottle with an ______ prep.

Answer 11

1, alcohol

Question 12

In smearing blood culture bottles, puncture the top of the bottle with a?

Answer 12

Safety venting Subculture unit

Question 13

In smears from blood culture bottles, allow __ drop to fall onto the slide and spread it until the liquid is about the size of a _______?

Answer 13

1, nickel

Question 14

In smearing from solid media, what is the first step?

Answer 14

Place a very small drop of H2O in the square

Question 15

In solid media smear, what is the size and type of syringe we should use?

Answer 15

1ml size, and tuberculin syringe with needle removed

Question 16

T or F: In smearing solid media, we rub the colony into the dry glass first then pull water into the smudge of organism, and mix until turbid.

Answer 16

True

Question 17

The use of a single stain to color a bacterial organism is commonly referred to as?

Answer 17

simple staining

Question 18

What are some of the most commonly used dyes for simple staining?

Answer 18

1. methylene blue
2. basic fuchsin
3. crystal violet

Question 19

The staining time for simple stains are?

Answer 19

30 seconds to 2 minutes

Question 20

What are the steps in simple staining?

Answer 20

1. Smear is stained with meth blue for 1 min
2. Wash with water
3. Water drops blotted with bibulous paper