SODIUM

Question 1

What is the major cation of extracellular fluid?

Answer 1

Sodium

Question 2

What are the functions of Sodium in the body?

Answer 2

Maintenance of the normal distribution of water
Osmotic pressure in the various fluid compartments

Question 3

What is the main source of body sodium?

Answer 3

Sodium chloride ingested in foods

Question 4

About how much total body sodium is contained in the skeleton?

Answer 4

1/3

Question 5

Hyponatremia is associated with what conditions?

Answer 5

Severe polyuria
Metabolic acidosis
Addison’s disease
Diarrhea
Renal Tubular disease

Question 6

Hyponatremia is associated with what conditions?

Answer 6

Hyperadrenalism
Severe dehydration
Diabetic coma after therapy with insulin
Excess treatment with sodium salts

Question 7

The present method of sodium colorimetric method is based on modifications of those first described by?

Answer 7

Maruna and Trinder

Question 8

In the first modification of Maruna and Trinder, sodium is precipitated as?

Answer 8

Triple salt

Question 9

Describe the reaction to produce chromophore

Answer 9

Sodium magnesium uranyl acetate with the excess Uranium is reacted with ferrocyanide.

Question 10

What is the filtrate reagent of the sodium colorimetric method?

Answer 10

Uranyl Acetate 2.1mM
Magnesium acetate 2.0mM in ethyl alcohol

Question 11

What is the acid reagent under SCM?

Answer 11

A diluted acetic acid

Question 12

What are the sodium colorimetric method?

Answer 12

Potassium ferrocyanide
Non-reactive stabilizers
Filters

Question 13

What is the sodium standard of the sodium chloride solution?

Answer 13

150mEq/L

Question 14

What is the storage of sodium reagent?

Answer 14

15-30C (Room temperature)

Question 15

The specimen that will be used should be?

Answer 15

Freshly drawn serum

Question 16

What is the stability of sodium?

Answer 16

24 hours
2 weeks if refrigerated

Question 17

What are the materials required but not provided?

Answer 17

Spectrophotometer
Centrifuge
Test tubes/rack

Question 18

In the laboratory activity, what is the volume of the filtrate reagent?

Answer 18

1000uL

Question 19

What is the volume of blk, std and sample added?

Answer 19

50 uL

Question 20

After the addition of the filtrate reagent, std, blk, and sample. What should be next?

Answer 20

Shake all the tubes vigorously for 3 minutes.

Question 21

What is the RPM of the centrifuge?

Answer 21

1500 for 10 minutes

Question 22

What is the volume of the acid reagent added?

Answer 22

1000uL

Question 23

What is the volume added of std,blk and sample supernatant?

Answer 23

50 uL

Question 24

What is the volume of the color reagent added?

Answer 24

50 uL

Question 25

At what absorbance are the tubes read?

Answer 25

550nm

Question 26

These elements have levels up to 3X normal and have no adverse influence on the procedure

Answer 26

Calcium, Chloride, Potassium

Question 27

This element have level up to 5X normal and have no adverse influence on the procedure

Answer 27

Phosphorus

Question 28

Failure to obtain the proper range of values in the assay of control may?

Answer 28

Indicate reagent deterioration Instrument malfunction Procedural errors

Question 29

Where is the sodium colorimetric compared to?

Answer 29

Flame photometric analysis