SPECIMEN COLLECTION Flashcards
URINALYSIS
o Specimen should be collected in a clear, clean, and dry container with a tight fitting lid.
o A regular voided specimen is acceptable for routine analysis. However, to avoid contamination of the specimen, a midstream collection is preferable.
o VOLUME: 20 cc to 50 cc
o STABILITY: 1hr
FECALYSIS
o Clear, non-breakable, sealable, and leak proof container
o AMOUNT: Peanut size
o STABILITY: 1hr
CSF AND OTHER BODY FLUIDS
o Specimen should be collected in a clear, dry, and sterile container
o STABILITY: Room temperature for 8hrs
SPERM ANALYSIS
o Abstains from sex or masturbation for several days before the test. (3-5 days)
o It is required to be collected in the morning
o Does not avoid ejaculation for more than 14 days before the test
o Avoid alcohol, caffeine, and marijuana before the sperm analysis
o Do not use lubricants when collecting the sample
o Clean, non-breakable, sealable, and leak proof container
o STABILITY: specimen must be processed immediately after submission
MICROBIOLOGY – URINE CULTURE & SENSITIVITY
o Urine for c & s must be collected in a sterile container
o A midstream clean-catch collection procedure ensures that the specimen is free of contamination from external genital areas.
o STABILITY: 2hrs room temperature
MICROBIOLOGY – BODY FLUIDS FOR C & S
o Specimen must be collected in a sterile container
MICROBIOLOGY – SPUTUM AFB
o Specimen must be collected in a sterile container
o Patient should not take any food or drinks before collecting the specimen
o Brushing of teeth is also not allowed
o Specimen must be collected early morning, deep cough for 3 consecutives days
o STABILITY: 2hrs room temperature
MICROBIOLOGY – GS, KOH, & WET SMEAR OF OTHER BODY FLUIDS
o Specimen must be collected in a sterile container or glass slide
o STABILITY: 2hrs room temperature
BLOOD CULTURE BOTTLE
o Aseptic draw
o VOLUME: 3cc to 5cc
HISTOPATHOLOGY – ROUTINE SUBMISSION
o All specimens must be placed in a secure, appropriately-sized, leak proof container, and covered with 10% buffered formalin or prefer.
o STORAGE: Room temperature
HISTOPATHOLOGY – FRESH SPECIMEN
o Fresh: no fixative must be used
o All fresh specimens must be transported to the laboratory STAT. (to know if benign or malignant)
o STORAGE: Room temperature
VACUTAINER TUBES – COLLECTION
o Follow order of draw
o Transport specimen on designated area
o Fill the required amount per tube
REQUISITION FORM
• Patient’s full legal name,
• Date of birth (DOB),
• Attending/Ordering physician,
• Date and time of collection,
• Specific test(s) requested or Specimen site and
source when required.SPECIMEN CONTAINER & TUBES
• LABEL INCLUDES:
o Patient’s full legal name,
o Date of birth (DOB),
o Date and time of collection,
o Specific site as appropriate for non-blood specimens (Microbiology, Histology, etc.)
o Label the container not the lid or biohazard bag
o When submitting glass slides, label the frosted end using a pencil with Patient’s full name and DOB
SPECIMEN REJECTION
- Specimen is not accurately and properly identified.
- Specimens that are received in an incorrect container, collected improperly.
- Specimens collected in expired collection media, tube, or kit.
- Insufficient specimen volume received to perform testing.
- They are not accompanied by a requisition/order.
- The quality/integrity of the sample is suboptimal or too old to yield accurate results
- Transported incorrectly
- Inadequate fixative
- Specimen too large for container
- Incorrect media
- Specimen stability compromised (age of specimen, temperature stored)
