Specimen collection and processing

Question 1

Heel stick is used for

Answer 1

Newborn screening

Question 2

Serum samples are collected in

Answer 2

Gold colored capped vacutainer tubes

Question 3

Plasma is the non cellular component of anticoagulated/coagulated blood gotten after ………………..

Answer 3

Anticoagulated
Centrifugation of the whole sample

Question 4

Serum samples are mostly used for running

Answer 4

Immunoassays

Question 5

Anticoagulants in Clinical Pathology labs

Answer 5

Heparin
EDTA
Sodium fluoride

Question 6

Heparin is available as lithium heparin and sodium heparin in

Answer 6

green capped vacutainer tubes

Question 7

Heparin accelerates the action of

Answer 7

Antithrombin III, preventing the formation of fibrin

Question 8

EDTA is mostly used in

Answer 8

Hematology tests and DNA studies

Question 9

EDTA is available in

Answer 9

Lavender capped vacutainer tubes

Question 10

Sodium fluoride inhibits what enzyme in glycolysis

Answer 10

Enolase enzyme

Question 11

Sodium fluoride is used for collecting samples for

Answer 11

Glucose assay

Question 12

Sodium fluoride is available in

Answer 12

Grey capped vacutainer tubes

Question 13

What anticoagulants form insoluble complexes with Calcium ions

Answer 13

Oxalates

Question 14

Oxalates are often mixed with

Answer 14

Fluoride in grey capped vacutainer tubes

Question 15

What anticoagulant inhibits glycolysis

Answer 15

Sodium iodoacetate

Question 16

Sodium Citrate is used for

Answer 16

Coagulation profile testing. Converts calcium to non ionized form

Question 17

Tubes of Sodium Citrate are

Answer 17

light blue capped vacutainer tubes

Question 18

Standard tubes used in chemistry labs are of two types

Answer 18

Vacutainer
Ordinary

Question 19

Match the Anticoagulants with the colors of the caps of their ordinary tubes

Answer 19

Lithium heparin- deep blue
EDTA- Green
Fluoride oxalate- Yellow
Plain tube- Red

Question 20

Order of draw

Answer 20

1. Sterile tubes
2. Coagulation study tubes
3. EDTA tubes
4. Heparin tubes
5. Fluoride oxalate
6. Serum sample tube

SCEHFS

Question 21

Interference can be as a result of

Answer 21

Haemolysis
Icteria
Lipaemia

Question 22

Lipaemia may be visible at Serum triglyceride concentration above

Answer 22

300mg/dl

Question 23

Lipaemic interferences can be reduced by

Answer 23

1. Ensuring at least 12hrs of fasting before sample collection
2. Ultracentrifugation of samples at speed of 10000g

Question 24

Icteric samples result when

Answer 24

Concentration of bilirubin is elevated

Question 25

To reduce the effect of spectral bilirubin interferences,

Answer 25

Run the sample blank alongside

Question 26

What analytes are affected by haemolysis

Answer 26

Potassium Phosphorus ALT Acid phosphatase

Question 27

What is used to reduce the effect of sample haemolysis

Answer 27

Sample blanking

Question 28

Samples for what essay should be protected from light

Answer 28

Bilirubin to prevent photo degradation

Question 29

Uncentrifuged samples should not be frozen before separation T/F

Answer 29

T To avoid haemolysis

Question 30

Anticoagulated blood samples should be centrifuged at what speed and time

Answer 30

15 minutes 2000-3000g

Question 31

Clotted whole blood should be centrifuged at what time and speed

Answer 31

30 minutes 1500g

Question 32

Samples should be lower the room temperature before the start of centrifuging T/F

Answer 32

F

Question 33

Specimens should be stored at what temperature

Answer 33

-20C, then -80C

Question 34

CSF is used to determine

Answer 34

Cell count, Glucose, Proteins estimation

Question 35

Establishing a reference interval would require how many individuals (REFERENCE INTERVALS)

Answer 35

120 to greater than 700

Question 36

Verifying a reference interval (transference) can require how many study individuals (REFERENCE INTERVALS)

Answer 36

20