Specimen collection & Media

Question 1

Multipurpose transport media

Answer 1

Stuart’s

Question 2

Contains selective agar (CA) & a CO2-generating tablet used for transporting Neisseria

Answer 2

JEMBEC system

Question 3

Absorbs fatty acids present in the specimen which is added to the media

Answer 3

Charcoal

Question 4

Fatty acid sensitive fastidious organisms

Answer 4

N. gonorrhoeae & B. pertussis

Question 5

Environment friendly preservative for stool

Answer 5

Polyvinyl Alcohol (PVA)

*Buffered formalin isnt eco friendly

Question 6

Organisms sensitive to higher concentrations of 0.025% SPS

Answer 6

G. vaginalis
Neisseria
S. moniliformis
P. anaerobius

Question 7

Anticoagulants for viral culture & mycobacterium

Answer 7

Heparin

Question 8

What anticoagulant/tube top is used for PCR testing in microbiology

Answer 8

White Top

Question 9

Storage temperature for urine, stool, viral spx, sputum & foreign devices

Answer 9

4°C

Question 10

Storage temperature for sterile body fluids, genital swabs, scrapings

Answer 10

22-25°C (RT)

Question 11

Storage Temperature for CSF and Blood in microbiology

Answer 11

37°C

Question 12

Storage temperature for tissues

Answer 12

-70°C

Question 13

Storage temperature for serum for serologic studies

Answer 13

-20°C

Question 14

Blood volume collected for microbiology

Answer 14

10-20 mL adults
5-10 mL children
0.5-1 mL infants (1-5 mL)

Question 15

Most abundant normal flora in upper respiratory tract

Answer 15

Alpha-hemolytic strep (Viridans)

Question 16

Most common pathogen in Upper respiratory tract

Answer 16

Beta-hemolytic strep (S. pyogenes)

Question 17

Contains broth with 0.075% agar & thioglycollate acid

Answer 17

Back-up broth

Question 18

Contains Schaedler broth with glycerol/chopped meat stored

Answer 18

Stock culture (for QC)

Question 19

Small zone of intact RBCs surrounded by a zone of Beta hemolysis after refrigeration

Answer 19

Alpha-prime

Question 20

Organisms that give off alpha prime on BAP

Answer 20

Viridans strep

Question 21

Inner zone of beta-hemolysis surrounded by a zone of alpha

Answer 21

Double hemolysis

Question 22

Organisms that give off double hemolysis on BAP

Answer 22

Clostridium perfringens

Question 23

Tap water agar is used to examine for organisms producing aerial hyphae

Answer 23

Nocardia
Streptomyces
Nocardiopsis

Question 24

Quantitative (cross-streaking) using calibrated loop (1:100/1000 uL) is used for?

Answer 24

Urine & tissue biopsy from burn px

Question 25

Semi-quantitation on inoculated plates is also called?

Answer 25

Isolation streaking

Question 26

Most commonly used method in measuring the number of viable cells in milk, food, water

Answer 26

Standard Plate Count

Question 27

Used for microscopic count of prokaryotes

Answer 27

Petroff-Hausser counter

Question 28

Inoculum size for Broth dilutions

Answer 28

5 x 10^5 CFU/mL

Question 29

Inoculum size for Agar dilutions

Answer 29

1.0 x 10^4 CFU/mL

Question 30

Disk diffusion Agar: Agar Depth: pH: Incubation: Inoculum:

Answer 30

Agar: Mueller-Hinton Agar Agar Depth: 4mm thick (3-5mm) pH: 7.2-7.4 Incubation: 16-24 hrs @ 35°C Inoculum: 1.5 x 10^8 CFU/mL

Question 31

Standard for disk diffusion

Answer 31

0.5 McFarland std (1% H2SO4 & 1.175% BaCl2)

Question 32

Disk storage

Answer 32

Long term: Non-frost/Frost Free freezer @ -20°C Short: 2-8°C for 1 week

Question 33

Distance for antibiotics in disk diffusion

Answer 33

24mm

Question 34

Oxacillin agar screen

Answer 34

Detection of STAPH resistance

Question 35

Oxacillin disk screen

Answer 35

Detection of STREP PNEUMONIAE resistance