Spectrophotometry

Question 1

What’s the role of a chromophore

Answer 1

responsible for light absorption, containing multiple double or triple bonds

Question 2

What’s the role of an auxochrome

Answer 2

modifying light absorption eg, hydroxyl group

Question 3

Molecules absorbing UV and visible light is dependent on

Answer 3

Bonding structure and functional groups present in the molecule

Question 4

Why is transmittance -log

Answer 4

This is because It is always smaller than i0 therefore the number is always smaller than one

Question 5

Equation for light Transmittance

Answer 5

T= It/I0

Question 6

What’s the relation between absorbance and transmittance

Answer 6

A= -logT = -log(it/i0)

Question 7

What is another way to measure absorbance

Answer 7

Using beer lambert

Question 8

What can the concentration of an analyte be determined by

Answer 8

1- measuring absorbance and using beer lambert
2-plotting reference curve of absorbance against conc

Question 9

What must the sample be to be to be able to use in a spectrophotometer

Answer 9

soluble in either aqueous or organic solvent

Question 10

If you change the pH to make the insoluble molecule soluble what does this mean?

Answer 10

The entire molecule is changed and so is the spec produced

Question 11

List 3 applications

Answer 11

pharmaceutical analysis
biopharmaceutical
forensic science