Spoilage Flashcards

(44 cards)

1
Q

When does spoilages start to become noticable

A

10^7 cells per gram

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2
Q

Generation time

A

time needed for the population to double

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3
Q

Which enzymes are present in aerobic MO’s that break down ROS?

A

superoxide dismutase and catalase

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4
Q

Obligate aerobe

A

Needs O2

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5
Q

Microaerophile

A

requires 2-0% O2, damaged by 20%

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6
Q

Facultative anaerobic

A

does not require O2, but grows better with it

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7
Q

aerotolerant anaerobe

A

grows equally well with or without O2

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8
Q

obligate anaerobe

A

Dies with O2 present

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9
Q

A lower pH gives a higher or lower redox potential?

A

higher Eh due to many H+

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10
Q

What happens when meat is vacuum packed?

A

There is residual respiration which consumes the O2 and produces CO2, this inhibits some MO’s.

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11
Q

Why does meat become pink/grey in a vacuum?

A

Because myoglobin can not bind to O2 anymore

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12
Q

xerophilic

A

grows on dry foods

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13
Q

osmophilic

A

grow in high concentrations of unionized compounds (sugar)

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14
Q

halotolerant

A

Grows in salted procts

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15
Q

Halophilic

A

Requires high levels of salt

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16
Q

What do microbicidal preservatives do?

A

Kill the MO’s

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17
Q

What do microbistatic preservatives do?

A

Prevent the growth of MO’s

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18
Q

Why would you add sulphite to a food

A

To acidify the product

19
Q

Why would you add nitrite to a product?

A

To target clostridium and for the colour of meat.

20
Q

psychrophile

A

Between -5 and -20 degrees

21
Q

Psychrotolerant

A

Between -5 and -35 degrees

22
Q

mesophile

A

Between 4 -47 degrees

23
Q

Thermophile

A

Between 40-90 degrees

24
Q

Thermotolerant

A

Will survive mild pasteurization

25
Competition between MO's
Growth of both MOs is suppressed, or one doesnt care, and the other one is suppressed
26
Symbiosis
Both need from eachother
27
Mutualism
Both benefit from each other
28
Commensalism
B benefits from A, A doesnt mind
29
Antagonism
A lowers growth of B
30
Challenge test
Inoculate product and follow the growth of the MO's
31
Storage test
Follow the fate of the product without spiking
32
Predictive microbiology
describe fate of MO by mathematical models
33
What is the average z value of vegetative cells?
5 degrees
34
Whats is the average z value of spores?
10 degrees
35
What is the lethal rate
the relative inactiviation rate at a certain temperature
36
F value
integrated lethal effect
37
What will happen after meat is slaughtered?
There will by glycoysis with the oxygen that is still in the animal. This will cause lactic acid production and inactivate glycolytic enzyme.
38
What will happen when you vacuum pack meat
Because of the low oxygen pseudomonas will not be able to grow, but LAB will take over. Meat will turn grey because of low oxygen in the myoglobin
39
What will happen to meat in MAP
Red colour will remain. CO2 will disolve into the meat at a low temperature, this makes the LAB more dominant.
40
What will happen to meat when you freeze it
There will be no bacterial growth due to the low aw and temperature, but the growth can continue when defrosting.
41
Why is fish so perishable?
Because of the high pH and because there is no post mortem acidification.
42
Which groups of MO's are targeted by vacuum packaging?
Anaerobic microorganisms
43
Which groups of MO's are targeted by heat treatment 90 degrees
Vegetative cells and non-resistant spores
44
Which groups of MO's are targeted by cooled storage
Mesophils and slows down growth of psychrotolerant MO's