Staining

Question 1

a. The name of the three-colour stains which identify the connective tissue.
b. Colours.

Answer 1

a. Masson’s trichrome and Mallory’s trichrome.
b. Green and blue.

Question 2

Weigerts stain:

Answer 2

Highlight elastic fibres - dark blue or purple.

Question 3

The name of the coloration which was used:

Answer 3

Massons trichrome and Mallorys trichrome.

Question 4

The name of the coloration which was used:

Answer 4

Orcein elastic stain - elastic fibers appear brown.

Question 5

Silver nitrate stain is used:

Answer 5

To identify:
a) the reticular fibers in connective tissue

b) neurofibrils in neurons
c) intercellular cement in epithelia

(brown/ black)

Question 6

Stained with:

Answer 6

Silver nitrate

Question 7

Sudan III or Sudan black

Answer 7

Used to stain lipids.

Question 8

The name of the staining is:

Answer 8

Sudan III or Sudan black

• for lipids.

Question 9

PAS (Periodic Acid Schiff) stain

Answer 9

Used to stain carbohydrates in pink (magenta)

• basement membrane.

Question 10

The coloration is:

Answer 10

(PAS) Periodic Acid Schiff

• stains carbohydrates in pink (meagenta)
• basement membrane.

Question 11

Iron hematoxylin

Answer 11

stains the muscle cells black

Question 12

Answer 12

Iron hematoxylin

• stains the muscle cells black

Question 13

The eight steps obtaining the histological section:

Answer 13

1. Obtaining the tissue
2. Fixation
3. Dehydration
4. Embedding
5. Sectioning
6. Staining
7. Mounting
8. Labeling