staining

Question 1

dye component that confers color by altering the light resonance properties of a compound

Answer 1

chromophore

Question 2

refers to a benzene containing chromophoric groups

Answer 2

chromogen

Question 3

group responsible for tissues to bind firmly to a dye

Answer 3

auxochrome

Question 4

collective term for synthetic/artificial (coal tar) dyes

Answer 4

aniline dyes

Question 5

combination of immunologic & histochemical techniques that allow phenotypic markers to be detected

Answer 5

immunohistochemical staining

Question 6

staining whereby various constituents of tissues are studied through chemical reactions that will permit microscopic localization

Answer 6

histochemical staining

Question 7

staining whereby tissue components are demonstrated in sections by direct interaction with a dye

Answer 7

histologic staining

Question 8

refers to selective staining of living cell constituents

Answer 8

vital staining

Question 9

recommended stain for mitochondria

Answer 9

Janus Green

Question 10

recommended stain for the reticuloendothelial system

Answer 10

Tryphan Blue

Question 11

vital staining by means of injecting the dye into any part of the animal

Answer 11

intravital staining

Question 12

vital staining by means of staining living cells immediately after removal from the living body

Answer 12

supravital staining

Question 13

best vital stain

Answer 13

neutral red

Question 14

selective stain for neutral fats

Answer 14

oil red O

Question 15

selective stain for hemosiderin

Answer 15

Perl’s Prussian Blue

Question 16

selective stain for polysaccharides

Answer 16

periodic acid schiff

Question 17

selective stain for elastic fibers

Answer 17

Weigert’s Elastic stain

Question 18

staining process that stains various tissue elements with little differentiation except between the nucleus and cytoplasm

Answer 18

routine staining

Question 19

staining is continued until the desired intensity of the color of the different tissue element is attained

Answer 19

progressive staining

Question 20

tissues are overstained and the excess dye is removed selectively until the desired intensity is obtained

Answer 20

regressive staining

Question 21

selective removal of excess stain to stain specific structures differentially from the rest of the surrounding tissue

Answer 21

differentiation/decolorization

Question 22

differentiator that acts by combining with the metal, breaking the latter’s union with the tissue or cell

Answer 22

acid differentiators

Question 23

differentiator that acts by oxidizing the dye to a colorless ubstance

Answer 23

oxidizing differentiator

Question 24

staining of tissues with a simple aqueous or alcoholic solution of the dye

Answer 24

direct staining

Question 25

staining of tissues with the addition of a mordant or accentuator to intensify the action of the dye

Answer 25

indirect staining

Question 26

substance that enhances the combination of the dye with the tissues

Answer 26

mordant

Question 27

substance that hastens the speed of intensity of the dye and has no chemical union between tissue and dye

Answer 27

accentuator

Question 28

substance that increases the rate of the staining action

Answer 28

accelerator

Question 29

process in which certain dyes stain certain tissues in a color that is different from that of the stain itself

Answer 29

metachromatic staining

Question 30

application of a different stain to provide contrast or background

Answer 30

counterstaining

Question 31

tree where hematoxylin is extracted from

Answer 31

Haematoxylum campechianum

Question 32

hematoxylin solution in which mordant and hematoxylin are mixed together

Answer 32

direct hematoxylin stain

Question 33

hematoxylin solution in which the mordant is not mixed together with the hematoxylin

Answer 33

mordant hematoxylin

Question 34

kind of eosin commonly used to compound Romanowsky stains

Answer 34

eosin B

Question 35

most common counterstain to alum hematoxylin in routine H&E

Answer 35

eosin Y

Question 36

effect of increasing dissolved salts (ionization) in the stain

Answer 36

decreased staining action

Question 37

effect of increased temperature to the stain

Answer 37

increased rate of penetration and staining action

Question 38

removal of excess paraffin so the stain can permeate the tissue sections

Answer 38

deparaffinization