Staining Flashcards
(19 cards)
What fixative must not be used before H&E staining method?
Osmic acid solutions
For tissues fixed with mercurice chloride, staining time of hematoxylin should be ____________ slightly while duration of eosin staining should be ____________.
increased; reduced
What is used to remove mercury?
Iodine-alcohol
What is used to remove iodine?
sodium thiosulfate and wash with water
The process of attaching sections by coating the side with dilute or thin celloidin.
Colloidionization
Production of formation of colors of different tissues and cells.
staining
Who attempted the use of saffron for staining?
Anton van Leeuwenhoek
Used carmine staining to diffuse colouration of tissues.
Goppert and Cohn
Who attempted the first use of selective nuclear stain.
Gerlach
What staining reaction occurs when tissue is penetrated by a dye solution?
absorption
What staining reaction is occurring when there’s an accumulation in the surface of the compound?
Adsorption
Sections are stained with a simple aqueous or alcoholic solution of the dye.
Direct staining
Requires the use of a mordant or accentuator in order to produce the desired density or intensity of color.
Indirect staining
A substance which serves as a link or bridge to form a colored lake.
Mordant
Accelerates or happens the speed of the staining reaction by increasing the staining power and selectivity of the dye.
Accentuator
Once the tissue is stained, it is not washed or decolorized. May result in diffused and obscuring effect.
Progressive staining
The tissue is first overstained to obliterate the tissue and excess stain is removed or decolorized.
Regenerative staining
Uses specific dyes which differentiate particular substances by staining them with a color different from that of the stain itself. It is dependent on the ability to polymerize.
Metachromatic staining
Application of a different color or stain to provide contrast and background to the staining of the structural components to be demonstrated.
Counterstaining
