STAINING

Question 1

The application of dyes on tissue sections to study the architectural patterns and physical characteristics of cells

Answer 1

Staining

Question 2

Affinity of nucleus is on _____ stains

Answer 2

Basic

Question 3

Affinity of cytoplasm is on ____ stains

Answer 3

Acidic

Question 4

Groups of Tissue staining

Answer 4

Histologic staining
Histichemical staining
Immunohistochemical staining

Question 5

A grouo of staining which has a direct interaction eith a dye or staining solution

Answer 5

Histologic

Question 6

A grouo of staining for the study if tissue constituentd through their chemicsl reactions

Answer 6

Histochemical staining

Question 7

A group of staining which is a combination of immunologic and histochemical techniques

Answer 7

Immunohustochemical staining

Question 8

A group of staining that detects phenotypic markers

Answer 8

Immunohistochemical staining

Question 9

Methods of Staining

Answer 9

1. Direct staining

2. Indirect staining

Question 10

A method of staining that uses a mordant

Answer 10

Indirect staining

Question 11

Term use to describe something that serves as a bridge or link between the tissue and the dye

Answer 11

Mordant

Question 12

It hastens the staining reaction by increasing the staining power and selectivity of dye

Answer 12

Accentuator

Question 13

A staining that is in definite sequence and with specific periods of time or until desired color is attained

Answer 13

Progressive staining

Question 14

Staining process that overstains

Answer 14

Regressive staining

Question 15

Staining with a color different from that of the stain itself

Answer 15

Metachromatic staining

Question 16

Staining used for contrast and background

Answer 16

Counter staining

Question 17

Selectively stsining of living cell constituents

Answer 17

Vital stains

Question 18

2 types of vital staining

Answer 18

Intravital staining and

Supravital staining

Question 19

Injecting the dye in any part of the animal body

Answer 19

Intravital staining

Question 20

Used after removal of stains from the living body

Answer 20

Supravital staining

Question 21

Best vital dye

Answer 21

Neutral red

Question 22

Term used for coloring substances

Answer 22

Stains

Question 23

2 categories of stains

Answer 23

Natural

Synthethic

Question 24

Natural dyes

Answer 24

1. Hematoxylin
2. Cochineal dyes
3. Orcein

Question 25

A natural dye extracted from coccus cacti or a female cochineal bug

Answer 25

Cochineal dyes

Question 26

Demonstrates a bright red color of glycogen

Answer 26

Cochineal dyes with aluminum chloride or Best Carmine

Question 27

Active coloring of hematoxylin

Answer 27

Hematin

Question 28

2 types of ripening

Answer 28

Natural Ripening | Artificial Ripening

Question 29

Ripening that exposes the substance to sunlight or air

Answer 29

Natural Ripening

Question 30

Ripening that uses chemical oxidation

Answer 30

Artificial ripening

Question 31

Natural dye extracted from Lichens

Answer 31

Orcein

Question 32

A natural dye that is source od Litmus paper

Answer 32

Orcein

Question 33

Aka Aniline dyes or Coal Tar dye

Answer 33

Synthethic dyes

Question 34

Substance added to chromogen by altering its shade that enables retaining of color

Answer 34

Auxochrome

Question 35

3 groups of Synthethic dyes

Answer 35

1. Acid dyes 2. Basic dyes 3. Neutral dyes

Question 36

A grou of synthetic dye that the coloring substance is found in acid component

Answer 36

Acid dyes

Question 37

A grou of synthetic dye that the coloring substance is found in basic component

Answer 37

Basic dye

Question 38

A group if synthethic dye that is formed by combining aqeous solutions of acid and basic dyes

Answer 38

Neutral dyes

Question 39

Routi ely used in H&E staining

Answer 39

Aluminum hematoxylin

Question 40

A staining solution that uses Iron salts as mordant and a ripening agent

Answer 40

Iron hematoxylin

Question 41

It is the standard Iron hematoxylin

Answer 41

Weigert's hematoxylin

Question 42

A hematoxylin stain for frozen sections

Answer 42

Loyez hematoxylin

Question 43

Mordant used in Tungsten hematoxylin

Answer 43

1% aqeous phosphotungstic acid

Question 44

Hematoxylin Stain for spermatogenesis

Answer 44

Copper hematoxylin

Question 45

A red acid dyeroutinely used as counterstain after hematoxylin and before methylene blue

Answer 45

Eosin

Question 46

3 forms of Eosin

Answer 46

1. Yellow (Eosin Y) - most common 2. Eosin B, erythrosin B - deeper red 3. Eosin S, ethyl eosin - soluble in alcohol

Question 47

Also known as Van Gieson's stain

Answer 47

Acid-Fuschin-Picric acid

Question 48

Also known as Masson stain

Answer 48

Acridine orange

Question 49

What color is DNA in acridine orange

Answer 49

Green fluorescence

Question 50

What color is RNA in acridine orange

Answer 50

Red fluorescence

Question 51

For staining hemoglobin

Answer 51

Benzidine

Question 52

Stain for blood, blood parasites, Borrelia

Answer 52

Giemsa stain

Question 53

Oldest stain

Answer 53

Iodine

Question 54

Used in removing mercuric fixative

Answer 54

Iodine

Question 55

2 types of iodine

Answer 55

1. Gram's iodine - microorganisms | 2. Lugol's iodine - glycogen, amyloid, corpora

Question 56

Stains for Fat

Answer 56

1. Oil soluble dyes or lysochromes 2. Sudan Black B - most sensitive 3. Sudan IV or Scharlach R - most commonly used 4. Sudan III - first Sudan Dye

Question 57

Other fat stains

Answer 57

Oil red O -neutral fats Osmic acid- unsaturated fats Nile blue sulfate method - differentiates two lipids

Question 58

Solvents used for staining

Answer 58

Water Alcohol Aniline water Phenol

Question 59

Stain for Negri Bodies

Answer 59

Schleifsteins

Question 60

First general stain for EM

Answer 60

Phosphitungstic acid

Question 61

Stain for M. Leprae

Answer 61

Farraco

Question 62

Stains for EM

Answer 62

Phosphotungstic acid Uranyl acetate Lead