Staining of Central Nervous System

Question 1

CNS

Answer 1

brain & spinal

Question 2

PNS

Answer 2

Nerves & ganglia

Question 3

What is under PNS

Answer 3

Somatic nervous system

Question 4

What is under CNS

Answer 4

Automic Nervous System

Question 5

______ which is associated with
voluntary body movement by the skeletal muscles.

Answer 5

SNS

Question 6

________ is a component that regulates involuntary processes such as heart rate, blood pressure, and respiratory rate. It is also where the flight or fight response is controlled.

Answer 6

ANS

Question 7

impulse conducting cells

Answer 7

neurons

Question 8

support and protects neurons

Answer 8

glial

Question 9

What are the glial cells

Answer 9

o Oligodendrocyte
o Astrocyte
o Ependymal Cell
o Microglia
o Schwann Cell

Question 10

Helps form the blood-brain barrier

Answer 10

astrocytes

Question 11

Replicates to occupy space of dying neurons

Answer 11

astrocytes

Question 12

Regulates interstitial fluid composition

Answer 12

astrocytes

Question 13

Provides structural support and organization to the
CNS

Answer 13

astrocytes

Question 14

Assists with neuronal development

Answer 14

astrocytes

Question 15

Lines ventricles of brain and central canal of spinal
cord

Answer 15

ependymal cells

Question 16

Assists in production and circulation of
cerebrospinal fluid (CSF)

Answer 16

ependymal cells

Question 17

Phagocytic cells that move through the CNS

Answer 17

microglial cells

Question 18

Protects the CNS by engulfing infectious agents and
other potential harmful substances

Answer 18

microglial cells

Question 19

Myelinates and insulates CNS axons

Answer 19

oligodendrocyte

Question 20

Allows faster action potential propagation along
axons in the CNS

Answer 20

oligodendrocytes

Question 21

Electrically insulates PNS cell bodies

Answer 21

satellite cells

Question 22

Regulates nutrient and waste exchange for cell
bodies in ganglia

Answer 22

satellite cells

Question 23

Surround and insulate PNS axons and myelinate
those having large diameters

Answer 23

Neurolemmocyte(schwann cells)

Question 24

Allows for faster action potential propagation along
an axon in the PNS

Answer 24

Neurolemmocyte(schwann cells)

Question 25

Also called as some or perikaryon

Answer 25

neuron - cell body

Question 26

It contains nucleus and surrounding cytoplasm

Answer 26

neuron - cell body

Question 27

- Large euchromatic nucleus with prominent nucleolus - chromatophilic substance (Nissl substance, Nissl bodies) basophilic regions consisting of polyribosomes and RER

Answer 27

neuron - cell body

Question 28

most neurons have a single _____

Answer 28

axon

Question 29

It arises from axon hillock

Answer 29

axon

Question 30

plasma membrane is called ______ whereas its contents are called ______

Answer 30

axolemma, axoplasm

Question 31

terminal branches of axons end in small button like structures called _________

Answer 31

presynaptic terminals

Question 32

Largest and most abundant neuroglia cells

Answer 32

astrocytes

Question 33

Fibrous long delicate processes, abundant in white matter

Answer 33

white matter

Question 34

Protoplasmic shorter processes, abundant in

Answer 34

gray matter

Question 35

When there is damage in the CNS, it is permanent because it cannot regenerate

Answer 35

reactive astrocytosis

Question 36

Respond to injury by producing a dense network of processes, somewhat analogous to fibrous scar that occurs elsewhere in the body.

Answer 36

Reactive astrocytosis

Question 37

In contrast to fibroblast, ______ do not produce collagen. Instead, the scar contains cytoplasmic processes.

Answer 37

Astrocytes

Question 38

Plasma membrane of the oligodendrocyte is wrapped around the ________

Answer 38

adjacent axons

Question 39

Produce and myelinate the axons in the cns

Answer 39

oligodendrocytes

Question 40

Single _________ can surround and myelinate several axons

Answer 40

oligodendrocyte

Question 41

_______ insulates axon and facilitates rapid transmission of nerve impulse

Answer 41

Myelin sheath

Question 42

● Smallest neuroglial cells ● Part of the mononuclear phagocyte system ● Part of immune defense of CNS

Answer 42

microglia

Question 43

Also called tigroid bodies

Answer 43

staiin of nissl bodies

Question 44

Made of rough ER with RNA in the cytoplasm of neurons

Answer 44

stain of nissl bodies

Question 45

Absence suggests _________

Answer 45

neuronal degeneration

Question 46

Can be stained with H&E but are best stained with Cresyl fast violet, thionine, toluidine blue and methylene blue

Answer 46

stain of nissl bodies

Question 47

Dissolution of the Nissl bodies in neurons

Answer 47

chromatolysis

Question 48

chromatolysis is usually triggered by

Answer 48

axotomy, ischemia, toxicity to the cell, and virus infections

Question 49

Its purpose is to identify the neurons in tissue sections or the demonstration of the loss of Nissl substance during chromatolysis

Answer 49

Cresyl fast violet (nissl) stain

Question 50

Section is covered with _______________

Answer 50

cresyl fast violet stain

Question 51

_______ appear purple to dark blue

Answer 51

Nissl bodies

Question 52

Neurons (cytoplasm) appear ______

Answer 52

pale purple

Question 53

fixation of cresyl fast violet stain

Answer 53

Alcohol, Carnoy's or neutral formol saline

Question 54

sections of cresyl fast violet stain

Answer 54

paraffin 7-10 um

Question 55

Cresyl fast violet nissl stain solutions

Answer 55

o Cresyl fast violet 0.5 gm. o Distilled water 100 ml. o Differentiating Solution o Glacial acetic acid 250 μl o Alcohol 100 ml.

Question 56

results of cresyl fast stain

Answer 56

▪ Nissl substance purple-dark blue ▪ Neurons pale purple blue ▪ Cell nuclei purple blue