Steg För Steg I Labben Flashcards by Oliver Karlsson

Q

Vad händer i steg 1?

A

Förbered bakterier i rör

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Q

Vad händer i steg 2?

A

Tillsätt bakterier från platta

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Q

Vad händer i steg 3?

A

Tillsätt plasmider (Cas9/gRNA)

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Q

Vad händer i steg 4?

A

Värmechock = plasmid tas upp

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Q

Vad händer i steg 5?

A

Tillsätt medium, vila

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Q

Vad händer i steg 6?

A

Sprid bakterier på plattor

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Q

Vad händer i steg 7?

A

Inkubera 24 h, 37 °C

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