Stem cell transplant

Question 1

BM collection has lower ______ content than PBSC and do not require mobilization

Answer 1

T cell

Question 2

Target cell dose for BM collection

Answer 2

2 x 10^8 TNC/kg

Question 3

Target cell dose for PBSC

Answer 3

> 5 X 10^6 CD34/kg

Question 4

HLA matching appears to demonstrate survival benefit at this stage of disease

Answer 4

early and intermediate

Question 5

risk of GVHD associated with HLA-DPB1 mismatching was influenced by the HLA-DPB1 rs9277534 expression marker, with _____ subtype being associated with greater risk than ______ subtype

Answer 5

G;A

Question 6

Melting Temperature equation

Answer 6

Tm= 4(G+C) + 2 (A+T)

Question 7

Temperature and salt conditions favored for high stringency

Answer 7

High and low

Question 8

PCR: temperature at which denaturation occurs

Answer 8

94-96C

Question 9

PCR: temperature at which primers anneal

Answer 9

50-65C

Question 10

PCR: temperature at which extention occurs

Answer 10

72C

Question 11

Too much Mg can decrease _______

Answer 11

fidelity

Question 12

Variable EDTA and Citrate in sample can effect amount of _____

Answer 12

MG

Question 13

Mg required for ______ activation

Answer 13

Taq polymerase

Question 14

KCl important in _______

Answer 14

primer annealing

Question 15

DMSO can be added and temperatures lowered when ____ content is high; however this can negatively affect_____

Answer 15

GC; Taq

Question 16

SSP requires ______ specificity

Answer 16

3’end

Question 17

Ideal primer length

Answer 17

~20 nucleotides

Question 18

Ideal primer GC content; however avoid ______

Answer 18

50%; GC runs

Question 19

Probe design: best specificity lies in ______ at the _____ of the probe

Answer 19

polymorphism; center

Question 20

Early CD3 engraftment may identify patients at risk of _____

Answer 20

GVHD

Question 21

How many cells do you need for 1 ng of template?

Answer 21

150

Question 22

How many cells do you need for 10 ng of template?

Answer 22

1500

Question 23

How many cell do you need for 100 ng of template

Answer 23

150,000

Question 24

Minimum dose of single cord transplant

Answer 24

3 x 10^7 TNC/kg

Question 25

http://hla.alleles.org

Answer 25

Nomenclature

Question 26

http://igdawg.org/cwd.html

Answer 26

Common and well documented alleles

Question 27

http://www.ebi.ac.uk/ipd/imgt/hla/

Answer 27

Allele history, alignments, serologic links

Question 28

https://bioinformatics.bethematchcli nical.org/

Answer 28

Allele/haplotype frequencies in US, haplotype tools, multi-allele codes, link to NMDP search strategy advice, CT policies

Question 29

http://www.pypop.org/popdata/

Answer 29

World-wide allele distribution maps

Question 30

http://www.bmdw.org/

Answer 30

World-wide database HLA phenotypes of volunteer donors, cbu for transplant centers/search coordinators; requires authorization to access

Question 31

National registries / cord blood banks; USA-- | https://bethematchclinical.org/

Answer 31

Preliminary & formal unrelated donor/cbu search (physician (prelim only), transplant center)

Question 32

https://www.mh-hannover.de/institute/transfusion/histocheck/

Answer 32

visualizing ribbon structure of HLA molecule and interrogation of peptide groove

Question 33

______ are rules mandated by the government

Answer 33

regulations

Question 34

______ are rules established by accrediting agency

Answer 34

standards

Question 35

STR: Mixture of donor and host

Answer 35

Mixed chimerism

Question 36

STR: One or more lineages complete donor others complete host

Answer 36

Split chimerism

Question 37

STR: Mixed chimerism in a subset (e.g., T cells)

Answer 37

Subset chimerism

Question 38

STR: <1% donor chimerism

Answer 38

microchimerim

Question 39

STRs are this long and are known as _______

Answer 39

2-8 basepairs(some variability); microsatellites

Question 40

VNTR are this long and are known as _______

Answer 40

>8 basepairs, usually much larger; minisatellites

Question 41

STR: Repeats that are short sequences of the same length

Answer 41

Simple

Question 42

STR: 2 or more adjacent repeats of same length

Answer 42

Compound

Question 43

STR: Variable length and composition repeats

Answer 43

Complex