Steps In DNA Replication

Question 1

STEP 2. Primer
Binding

Once the DNA strands have been separated, a short piece of RNA
called a _______ binds to the _____’ end of the strand.

Primers are generated
by the enzyme _______.

Answer 1

primer, 3’
Primase

Question 2

DNA replication, both strands are replicated in a
_________ manner

Answer 2

Semiconservative

Question 3

STEP 1. Replication ______
Formation

Before DNA can be replicated, the doublestranded molecule must be “unzipped” into two ______ strands.

Interactions between base pairs are broken down by an enzyme known as ________

Answer 3

Fork
Single strand

Question 4

STEP 1. Replication
Fork Formation

DNA helicase disrupts the hydrogen bonding between base pairs to separate the strands into a Y shape known as the ________.

This area will be the
template for ______ to begin.

Answer 4

Replication fork
Replication

Question 5

STEP 3. Elongation

Enzymes known as DNA
________ are responsible creating the
new DNA strand.

DNA polymerase ____ begins synthesizing DNA in the _______ direction.

Take note! The DNA polymerase cannot function without attaching first to the 3’ end of the RNA primer

Answer 5

polymerases
III
5′→3′

Question 6

STEP 3. Elongation

The newly synthesized strand is complementary and _______ to the parental strand used as a template.

Because replication proceeds in the 5’ to 3’ direction on the _______
strand, the newly formed strand is ________

Answer 6

antiparallel
leading
continuous

Question 7

STEP 3. Elongation
The _______ strand begins replication by binding with multiple
primers

DNA polymerase then adds pieces of DNA, called _________ fragments, to the strand between primers.

This process of replication is
_______ as the newly created fragments
are disjointed

Answer 7

lagging
Okazaki
discontinuous

Question 8

STEP 4. Termination

Once both the continuous and
discontinuous strands are formed, an enzyme called ________ removes all RNA primers.

Another enzyme called DNA ______
joins Okazaki fragments together forming a single unified strand.

Once completed, the parent strand and its complementary DNA strand coils into the familiar _______ helix shape.

In the end, replication produces _______ DNA molecules, each with one strand from the parent molecule and one new strand

Answer 8

exonuclease
ligase
double
Two

Question 9

______ are sections of DNA found at the ends of each chromosomes.

They consist of the same sequence of bases repeated over and over.

In humans the telomere
sequence is ______.

Answer 9

Telomeres
TTAGGG