Sterilization Flashcards by Ellen Bravo

Biologic Agents that are acquired by ingestion, percutaneous exposure

Biosafety level 2

How well did you know this?

Not at all

Perfectly

Chamber filled with hot steam under pressure

Autoclave

How well did you know this?

Not at all

Perfectly

Removal or destruction of all forms of life

Sterilization

How well did you know this?

Not at all

Perfectly

Most commonly used method for the removal of microorganisms

Heat

How well did you know this?

Not at all

Perfectly

Biological indicator of autoclave

Bacillus stearothermophilus

How well did you know this?

Not at all

Perfectly

Temperature, psi for decontaminating medical waste using autoclave

132C 15psi for 30mins

How well did you know this?

Not at all

Perfectly

Destroys vegetative or non sporulating bacteria

Boiling

How well did you know this?

Not at all

Perfectly

Instrument used in tyndallization

Arnold’s sterilizer or free flowing steam

How well did you know this?

Not at all

Perfectly

Fastest and simplest method of sterilization

Autoclave

How well did you know this?

Not at all

Perfectly

Temperature and time of exposure for boiling

100C for 10-15mins

How well did you know this?

Not at all

Perfectly

Temperature and time of exposure for tyndallization

100C for 30mins in 3 consecutive days

How well did you know this?

Not at all

Perfectly

Used to sterilize protein rich media. Such as LJ

Inspissation

How well did you know this?

Not at all

Perfectly

Used to sterilize milk, dairy products and alcoholic beverages

Pasteurization or partial sterilization

How well did you know this?

Not at all

Perfectly

Temperature, psi and minutes to sterilize media, liquids, glass pipets and instruments for assay using autoclave

121C, 15psi for 15 mins

How well did you know this?

Not at all

Perfectly

Temperature and time of exposure for inspissation

70-80C for 2hrs in 3 consecutive days

How well did you know this?

Not at all

Perfectly

This method cannot eliminate bacterial endospores

Pasteurization

How well did you know this?

Not at all

Perfectly

Treatment at this temperature reduces spoilage if food without affecting its taste

Low temperature holding or

Batch method

How well did you know this?

Not at all

Perfectly

Utilized for sterilization of glasswares, oil products and powders

Dry heat procedure-oven

How well did you know this?

Not at all

Perfectly

Destroys milk pathogens

Batch method

How well did you know this?

Not at all

Perfectly

Temperature and time of exposure for batch method

63C for 30mins

How well did you know this?

Not at all

Perfectly

Temperature and time of exposure for flash pasteurization

72C for 15sec

How well did you know this?

Not at all

Perfectly

Cooled very quickly in a vacuum chamber

Ultra high temperature

How well did you know this?

Not at all

Perfectly

Milk can be stored at room temperature for two months without affecting its flavor

Ultra high temperature

How well did you know this?

Not at all

Perfectly

Principle of inspissation

Thickening of media through evaporation

How well did you know this?

Not at all

Perfectly

Temperature and time exposure for ultra high temperature

140C for 3sec

Not applicable for heat sensitive material

Dry heat procedure

Biological indicator of Dry heat procedure

Bacillus subtilis var niger

Direct heating

Flaming

Temperature and time of exposure for oven

160-170C for 1.5-2hrs

Sterilization of heat sensitive solution

Filtration

Kills microorganisms by denaturation of proteins

Dry heat procedure

Most common method of treating infectious waste and infected laboratory animals

Incineration

Principle of incineration

Burning materials into ashes in 300-400C

Potential biologic agents for aerosol transmission, lethal pathogens

Biosafety level 3

Used to control the spread of communicable diseases

Cremation

Refers to the lowest temperature at which a suspension of bacteria is killed in 10mins

Thermal death point

Shortest period of time needed to kill a suspension of bacteria at a prescribe temperature and under specific conditions

Therrmal death time

The time in a minute to reduce the bacterial population or spores by 90% at a specified temperature

Decimal reduction time

Quick heating then immediate cooling

High temperature short time Or Flash pasteurization

Two types of filter

Depth and membrane filters

Destroys bacteria by changes in proteins and decreased chemical reactions

Lyophilization

Method of choice for sterilization of antibiotic solutions, toxic chemicals, radioisotopes, vaccines

Filtration

Consists of fibrous or granular material

Depth filters

Easily transmitted and highly infectious agent of bioterrorism

Category A

These are porous membranes almost 0.1mm thick. Composed of cellulose acetate or polycarbonate

Membrane or circular filter

Uses cellulose acetate or cellulose nitrate with a vacuum

Liquid filtration

Uses HEPA filer

Air filter

Used to sterilized biohazardous trash and heat stable objects

Autoclave

Remove organisms larger than 0.3um from isolation rooms, OR and biological safety cabinet

High efficiency particulate air (HEPA) filter

Destroys bacteria by disruption of metabolism

Dessication

Destroys microorganisms by coagulation of enzymes and structural proteins and degradation of nucleic acid

Moist heat procedure

Bacteriostatic. | Reduces the rate of metabolism

Low or cold temperatures

Bacterias that can withstand dryness

N. gonorrheae (1hr) MTB- several months Bacillus and clostridium 10yrs

Pore size of membrane filters that remove most bacteria as well as fungi but not viruses

0.2-0.45um

Uses 0.22um membrane filters for parenteral solution

Critical sterilizing

Membrane filter pore size that used to remove vegetative cells

0.22um

Important in food microbiology

Low or cold temperatures

Involves removing water from microbes

Dessication

Most commonly used BSC in clinical lan

Class IIA

Most effective method for long term preservation of microbial cultures

Lyophilization

Used in the form of hypochlorite

Chlorine

Turning culture into powder

Lyophilization

Use of high concentrations of salts and sugars in food create a hypertonic environment

Osmotic pressure

Biological indicator of radiation

Bacillus pumilus

Causes mutation in DNA and produce peroxidase

Ionizing radiation or cold sterilization

Treponema pallidum is killed when exposed to

2-8C for 72hrs

Biologic agents that Include all common agents of infectious diseases

Biosafety level 2

Applied topically to skin

Antiseptic

Destroys vegetative cells and endospores of both prokaryotes and eukaryotes

Ionizing radiation

Examples are B. Subtilis and N. Gruberi

Biosafety level 1

Also used to "pasteurize" meat products

Ionizing radiation

Causes damage to cellular DNA by producing thymine dimers

Non ionizing radiation

Refers to removal, inhibition or killing of microorganisms including potential pathogens by using chemical agents usually on inanimate objects

Disinfection

Applied to inanimate object

Disinfectant

Dangerous and exotic biologic agent. Cause life threatening infection

Biosafety level 4

Should be allowed to evaporate from the surface to which they were applied to achieve maximum effectivity

Alcohol

Biologic agent that Include those that have no known potential for infecting healthy people

Biosafety level 1 agents

Bioterrorism agent with moderate morbidity and low mortality

Category B

When it passes through the cells, it creates free hydrogen and hydroxyl radicals and some peroxidase which in turn cause different intracellular damage

Radiation

It kills all bacteria in the specimen

Bactericidal

Hydrolyzes and coagulates proteins

Acid and alkaline solution

Destroys microorganisms by oxidation mechanism

Halogen

First widely used antiseptic and disinfectant

Phenol

For cleaning blood spills

5.25% hypochlorite

Combination of iodine and neutral polymer

Iodophors

Betadine must be in contact with an object for how long

1-2mins

Destroys microorganism by inactivation and precipitation of cell proteins

Salts of heavy metals

Eye drop solution.

AgNO3

Bioterrorism agent. Emerging pathogens

Category C

Widely used as surface active agents

Detergent

Provides most protection to worker

Class III cabinet

Bacteria that is resistant to detergent

Pseudomonas aeruginosa

Sterilization without water

Dry heat procedure

Sterilizes HEPA filter in BSC

Formaldehyde

Highest dilution that kills bacteria after a 10mins exposure

Phenol coefficient

Used to disinfect drinking water

Halozone

Pose the greatest public health threat

Category A

Has a rapid killing action but does not penetrate organic material

Glutaraldehyde

Also known as laminar flow BSC

Class II cabinet

Biologic agent Used in lab teaching exercises

Biosafety level 1

Commonly used gas for sterilization

Ethylene oxide

Inhibits the growth of organism

Bacteriostatic

Biological indicator of EtO

Bacillus subtilis var. globijii

More stable than iodine in its pure form

Iodophor

Active against all vegetative microorganisms and fungal spores

Periacetic acid

PC result that indicates that the disinfectant is more effective than phenol

PC>1

Device that encloses a working area to protect workers from aerosol exposure

Biological safety cabinet

Open fronted type of cabinet with negative pressure

Class I cabinet

Used to sterilize heart lung machine

Ethylene oxide

May e used with both liquid and air

Filtration

Principle f autoclave

Steam under pressure

Sterilization Flashcards

(111 cards)