Structural Chain

Question 1

Examples of structural stains

Answer 1

Capsule
Endospore
Flagella
Granules
Cell wall

Question 2

Maintain the shape of peptidoglycan

Answer 2

Cell wall

Question 3

Staining technique for cell wall.

Answer 3

Dyar’s stain

Question 4

Components of Dyar’s stain?

Answer 4

Mordant: cetyl pyridium chloride
Congo red

Question 5

T or F
In Dyar stain, + cell wall stains pink

Answer 5

False- red

Question 6

It stored phosphates in some bacteria

Answer 6

Metachromic granules

Question 7

Corynebacterium diptheriae
appears as
___.

Answer 7

“ X”, “Y” or chinese characters

Question 8

Which are not part of metachromic granules staining?
A. Lamb, Neisser, Ljubinsky
B. Gohar,metylene blue
C. Ponder, albert
D. Acid fast, gram stain

Answer 8

D

Question 9

Organelle of locomotion

Answer 9

Flagella

Question 10

All are staining methods of flagellar stain excpept..
A. Leifson, silver plating
B. Gohar and albert
C. Fisher and Conn
D. Leifson and Hugh

Answer 10

B

Question 11

What is the mordant in flagellar stain?

Answer 11

Tannic acid

Question 12

T or F
Flagella staining needs heat fixing

Answer 12

False

Question 13

Common stain in cell wall?

Answer 13

Dyar’s stain

Question 14

• Dyar’s stain ( Mordant)

Answer 14

cetyl pyridium chloride

Question 15

Dyar’s primary stain
Congo red

Question 16

Positive cell wall stains what color in Dyar’s stain?

Answer 16

Red

Question 17

Most common stains in metachromic granules.

Answer 17

Methylene blue

Question 18

Mordant stain in flagella staining.

Answer 18

Tannic acid

Question 19

-acts as anti phagocytic shield

Answer 19

Capsule

Question 20

-are organized, fixed structures to the cell wall
-polysaccharide + glycoprotein layer

Answer 20

Capsule

Question 21

gelatinous substance that is not fixed to the cell wall and not highly organized

Answer 21

Slime layer

Question 22

-produces grape like odor/tortilla like

Answer 22

Pseudomonas aeruginosa

Question 23

Common capsule stain

Answer 23

Anthony’s stain

Question 24

Negative staining

Answer 24

India ink

Question 25

Responsible for survival of bacteria during adverse condition

Answer 25

Endospore

Question 26

Different endospore stain

Answer 26

1. Heat and Acetic acid 2. Abbott 3.Dorner 4. Wirtz and Conklin 5.Schaeffer and Fulton

Question 27

Primary stain i Schaeffer fulton

Answer 27

5% malachite green .

Question 28

In schaeffer fulton how long does it takes to steam.

Answer 28

5 mins.

Question 29

Counter stain (30s-60s)

Answer 29

Safranin

Question 30

(+) red spores against gray background

Answer 30

Dorner stain