Structural Properties of Specific Proteins

Question 1

Triose Phosphate Isomerase (TIM)

Answer 1

• domain that occurs in many proteins
• central Beta barrel that’s surrounded by alpha helices
• 8 fold repeated beta-alpha motif

Question 2

Trypsin

Answer 2

• all Beta protein
• catalytic triad: Ser 195, His 57, Asp 102

Question 3

Chymotrypsin prefers…

Answer 3

large hydrophobic side chains

Question 4

Trypsin prefers

Answer 4

Lysine or Arginine

Question 5

Elastase prefers

Answer 5

Alanine, Glycine, Valine side chains

Question 6

Ribonuclease A

Answer 6

• active site is sandwiched between 2 Beta sheets
• 2 histidines carry out the acid-base catalysis
  -Lysine helps stabilize the intermediate state
• all Beta protein with 4 disulfide bridges