T4L2 - Medical Microbiology

Question 1

Bacteria [4]

Answer 1

Small, single-celled, prokaryotic organisms

No distinct nucleus

Transcription & translation carried out simultaneously

Divide by binary fission, may be mobile

Question 2

Bacteria examples [3]

Answer 2

Spirochetes, rickettsia & actinomyces

Question 3

Viruses [2]

Answer 3

Particles of nucleic acid DNA or RNA
- In a protein coat
+/- a lipid capsule

Question 4

Black plague

Answer 4

Yersina Pestis 1/3 Europe

Question 5

Mycobacterium tuberculosis

Answer 5

1:4 UK

18-19th century

Question 6

Peptidoglycans [4]

Answer 6

3D polymer
N-acetylated sugars
3-5 AA
Transpeptidase cross links

Question 7

N-acetylated sugars [2]

Answer 7

glucosamine (NAG) and muramic acid (NAM)

Question 8

Mycobacteria [3]

Answer 8

Gram positive cell wall

Don’t stain gram positive

Very thick lipid membrane anchored to peptidoglycans layer (intracellular survival)

Question 9

Staining

Answer 9

Dry on glass plate 
Crystal violet 
Set with iodine 
Decolourise with alcohol 
Counterstain with Safranin 
Purple = +ve

Question 10

lack cell wall

Answer 10

Mycoplasma

Chlamydia

Question 11

Phases

Answer 11

Lag
Exponential
Stationary
Death

LESD