TA Session -1 (Cullen) Flashcards by Zee Daws

Bacteria have with of the following ribosomes?

A. 60s
B. 70s
C. 80s
D. 90s

B. 70s

Note: 80s = eukaryotic cells

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Bacteria contain a nucleus

True or False?

False

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Which of the following do bacteria have

A. Sterols
B. Nucleus
C. Histones
D. Complex cell wall

D. Complex cell wall

Note: They don’t have the other answer choices

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Which type of bacteria is known for having a thick layer of peptidoglycan and teichoic acid?

A. Gram +
B. Gram -
C. Mycobacteria

A. Gram +

Note: teichoic acid (specific to gram +)

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Which of the following is known for having thin peptidoglycan and LPS?

A. Gram +
B. Gram -
C. Mycobacteria

B. Gram -

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Which of the following is known to have mycolic acid ?

A. Gram +
B. Gram -
C. Mycobacteria

C. Mycobacteria

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Which of the following is an endotoxin?

A. Specific O chain in Gram - species
B. Lipid A portion of Gram - species
C. A toxin produced inside Gram + species by normal metabolic actively
D. Lipid A portion of Gram + species

B. Lipid A portion of Gram - species

Note: Exotoxins are: A toxin produced inside Gram + species by normal metabolic actively

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The death phase (of bacteria) can lead to the death of the patient (gram -)

True or False ?

True

Note: not very likely

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What tests for endotoxin?

A. CBC
B. Blood Biochemistry
C. PT/PTT
D. Limbus amebocyte lysate test

D. Limbus amebocyte lysate test

Note: horse-shoe crab slide

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Which of the following cannot utilize oxygen for growth

A. Aerobic
B. Faculatative anaerobe
C. Capnophilic
D. Aerotlerant anaerobe

D. Aerotlerant anaerobe

Note: Can grow in the presence of oxygen but it will not utilize oxygen for growth

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Virulence is the ability of a pathogen to produce a disease by overcoming the defense mechanism of the host ?

True or False

False

Note: Pathogenicity would be correct here

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Endotoxins are metabolic products of gram + species and exotoxins are the lipid A portion of outer membrane of gram - species

True or False

False

Note: Reverse Endotoxins & exotoxins for correct statement

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Which of the following is not a method of transferring virulence factors from one bacteria to another?

A. Mutation
B. Transduction
C. Conjucation
D. Transformation

A. Mutation

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After culture and sensitivity the best antimicrobial to use will be under which break point

A. Lethal
B. Resistant
C. Intermediate
D. Susceptible

D. Susceptible

Note: Lethal is not a break point (there is only 3)

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Which of the following is not a reason to culture and sensitivity testing?

A. Save time
B. Prevent disease relapse
C. Better treat your patients
D. Prevent antimicrobial resistance

A. Save time

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Which of the following cannot be used to determine MIC?

A. E-Test
B. Kirby Bauer disk diffusion
C. Both dilution
D. Gradient diffusion test

Kirby Bauer disk diffusion

In regards to specificity and sensitivity an ideal test has

A. High sensitivity; low specificity
B. Low sensitivity; low specificity
C. Low sensitivity; low specificity
D. High sensitivity; High specificity

High sensitivity; High specificity

Acid fast + organisms appear pink while Acid fast - organism stain blue

True or False?

True

When collecting tissue/samples from necropsy for microbial testing, these samples should be collected last due to personal safety issues

True or False?

False

Note: Want to collect it immediately

PEA agar is selective for gram - species while McConkeys agar is selective for gram + species

True or False?

False

Note: It’s the other way around; McConkeys is always gram - …etc.

When an antimicrobial inhibits further replication of bacteria but does not kill the bacteria it is referred as a bacteriocidal antimicrobial

True or False

False

Which of the following uses mass spectrometry for microbial identification?

A. PCR
B. Interferon Gamma Test
C. Antibody fluoresence
D. MALDI-TOF

D. MALDI-TOF

Staphylococcal species which are coagulase negative (such as S. felis) are more virulent than species which are coagulase positive (S. hyicus)

True or False?

False

Staphylococcus species contain a virulence factor, Protein A, which binds to the FC portion of IgG. Once this happens further binding to complement or macrophage cannot happen

True or False?

True

Bumble foot in birds is caused by which agent? A. Staphylococcus hyicus B. Staphylococcus intermedius C. Staphylococcus aureus D. Staphylococcuspseudintermedius

C. Staphylococcus aureus

On a culture and sensitivity a species of Staph appears susceptible to Clindamycin but resistant to erythromycin (Macrolide). Should you immediately treat with Clindamycin? A. Yes B. No

B. No Note: You have to do a D-test before you can treat with Clindamycin

The catalase test can be used to distinguish between Streptococcus and Staphylococcus species. Which species is catalse positive? A. Staph B. Strep

A. Staph

Streptococcus is faculatative anaerobic meaning (depending on the exact species) it does not require oxygen, but if oxygen is present it can be utilized for metabolism True or False?

True

Lancefield testing amongst Streptococcus species classifies species based on cell wall proteins True or False?

False Note: .... based on Carbohydrates

Which Streptococcus group is most common in canine and feline infections A. G B. B C. C D. D

You have graduated RUSVM and now working in epidemiology as an infectious agent investigator. There has been a break out of Fishermen becoming sick and you are asked to see if there is some sort of zoonotic link. You perform multiple necropsies on the fish that the men bring in. You not bacteria in the gills, within the bloodstream, and some cases even find it in the CNS. For further testing you will need a top differential to hopefully rule in/out. What is your top differential? A. Staph. aureus B. Staph. iniae C. Staph. intermedius D. Strep. iniae

D. Strep. iniae

Which Streptococcus species follows the one speies, one host method? S. equi S. canis S. zooepidemicus All of the above

S. equi

What is unique about the capsule of Streptococcus A. Teichoic acid B. N-acetyl muramic acid C. N-acetyl glucosamine D. Hyaluronic acid

D. Hyaluronic acid

Bastard strangles is disseminated strangles infection an purpura hemorrhagica is an immune complex medicated vasculitis. Both of which are secondary to Strep equi subsp equi infection True or False?

True

When diagnosing Streptococcus using PCR one should target ? DNA sequence RNA sequence M Protein gene Hyaluronic acid capsule gene

M Protein gene

You are managing a feed lot and brought some new silage at what seemed to be too good of a deal. Sure enough a week later you start noticing problems. 50% of the stock have become diseased. The biggest symptom noticed so far is the animal walking in circles. What is your top differential? A. Staph. aureus B. Strep. equi C. Erysipeolothrix tonsillarum D. Listeria monocytogenes

D. Listeria monocytogenes

Which of the following is most important in regards to immune system fighting of listeria infection A. Humoral immunity B. Cell mediated immunity

B. Cell mediated immunity

E. rheusiopathiae is associated with disease in pigs where as E. tonsillarum is considered avirulent on pigs but has been associated with disease in dogs True or False ?

True